Resveratrol improves endothelial cell markers impaired by plasma incubation from women who subsequently develop preeclampsia

被引：0
作者：
Mayara Caldeira-Dias
Marcelo F. Montenegro
Heloisa Bettiol
Marco Antonio Barbieri
Viviane C. Cardoso
Ricardo C. Cavalli
Valéria C. Sandrim
机构：
[1] Institute of Biosciences of Botucatu,Department of Pharmacology
[2] Universidade Estadual Paulista (UNESP),Department of Physiology and Pharmacology (FYFA)
[3] Distrito Rubiao Junior,Department of Pediatrics
[4] Karolinska Institutet Biomedicum,Department of Gynecology and Obstetrics
[5] Faculty of Medicine of Ribeirao Preto,undefined
[6] University of Sao Paulo,undefined
[7] Faculty of Medicine of Ribeirao Preto,undefined
[8] University of Sao Paulo,undefined
来源：
Hypertension Research | 2019年 / 42卷
关键词：
Endothelial cells; Plasma; Preeclampsia; Resveratrol;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
In this study, we demonstrated that plasma collected from women who subsequently developed preeclampsia caused increased heme oxygenase-1 (HO-1) production and decreased levels of nitric oxide (NO) markers in endothelial cells (HUVECs). Conversely, no changes in HO-1 or NO markers were found when HUVECs were treated with plasma from women who remained healthy throughout pregnancy. These alterations in HO-1 and NO markers were prevented by cotreatment with the polyphenol resveratrol, which also improved GSH levels. In addition, we evaluated changes induced by plasma incubation in the expression of genes and their related pathways associated with antioxidant defenses, such as Nrf2, ARE activity, and GSR. Collectively, our findings suggest that even before the appearance of clinical symptoms of preeclampsia, plasma from affected women is able to induce modifications in endothelial cells with respect to HO-1 production and NO markers. We believe that this in vitro strategy may offer an attractive alternative to the exploitation of candidate markers or screening molecules, such as resveratrol, for the prevention and management of preeclampsia.
引用
收藏
页码：1166 / 1174
页数：8
相关论文
共 269 条
  • [1] Duley L(2009)The global impact of pre-eclampsia and eclampsia Semin Perinatol 33 130-7
  • [2] Hutcheon JA(2011)Epidemiology of pre-eclampsia and the other hypertensive disorders of pregnancy Best Pract Res Clin Obstet Gynaecol 25 391-403
  • [3] Lisonkova S(2014)Management of preeclampsia Pregnancy Hypertens 4 246-7
  • [4] Joseph KS(2016)Therapeutically targeting mitochondrial redox signalling alleviates endothelial dysfunction in preeclampsia Sci Rep 6 53-7
  • [5] Dekker GA(2002)Serum from healthy pregnant women reduces oxidative stress in human umbilical vein endothelial cells Clin Sci 103 1-5
  • [6] McCarthy C(2017)Oxidative stress in pre-eclampsia; have we been looking in the wrong place? Pregnancy Hypertens 8 194-203
  • [7] Kenny LC(2010)Arginase contributes to endothelial cell oxidative stress in response to plasma from women with preeclampsia Cardiovasc Res 85 279-86
  • [8] Scalera F(2013)Inhibition of lectin-like oxidized low-density lipoprotein-1 receptor protects against plasma-mediated vascular dysfunction associated with pre-eclampsia Am J Hypertens 26 457-64
  • [9] Fischer T(2017)The NOX2-derived reactive oxygen species damaged endothelial nitric oxide system via suppressed BKCa/SKCa in preeclampsia Hypertens Res 40 21-5
  • [10] Schlembach D(2018)Epigallocatechin gallate enhances treatment efficacy of oral nifedipine against pregnancy-induced severe pre-eclampsia: A double-blind, randomized and placebo-controlled clinical study J Clin Pharm Ther 43 595-600
12345678910