The Diagnostic Potential of Monoclonal Antibodies to Adenovirus

被引：0
作者：
T. A. Timoshicheva
I. V. Amosova
M. P. Grudinin
机构：
[1] Smorodintsev Research Institute of Influenza,
[2] Ministry of Health of the Russian Federation,undefined
来源：
Applied Biochemistry and Microbiology | 2022年 / 58卷
关键词：
monoclonal antibodies; human adenovirus; adenovirus infection; diagnosis;
D O I：
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学科分类号：
摘要：
The diagnostic potential of the 4B7 and 6B12 monoclonal antibodies (MAbs) against human adenovirus hexon protein has been studied in various immunological tests, namely, the in-cell enzyme-linked immunosorbent assay (cell-ELISA), sandwich ELISA, and the immunofluorescence antibody technique (IFA). It was shown that the sensitivities of cell-ELISA, sandwich ELISA, and IFA were 96, 86, and 84%, respectively, compared to PCR. Thus, 4B7 and 6B12 MAbs are promising immunoreagents for the construction of various diagnostic kits to use in laboratory practice for adenovirus detection in clinical samples.
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页码：873 / 877
页数：4
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共 46 条
[1]  
Walter J.M.(2017)Severe respiratory viral infections: new evidence and changing paradigms Infect. Dis. Clin. North Am. 31 455-474
[2]  
Wunderink R.G.(2015)Adenoviruses in the etiological structure of acute respiratory viral infections in Moscow in 2004–2014 Zh. mikrobiol., epidemiol. immunobiol. 21 50-57
[3]  
Yatsyshina S.B.(2013)Respiratory infections in the military J.R. Army Med. Corps. 159 181-189
[4]  
Ageeva M.R.(1991)Prognostic factors in childhood acute encephalitis Pediatr. Infect. Dis. J. 10 441-446
[5]  
Vorob’eva N.S.(2019)Use of hexon as an antigen for the production of monoclonal antibodies capable of detecting multiple adenovirus types Biologicals 58 44-49
[6]  
O’Shea M.K.(2018)Molecular genetic analysis of modern adenovirus strains Sovr. Med. 3 13-16
[7]  
Wilson D.(2017)The use of microcultural enzyme immunoassay and a modified immunofluorescence method for the diagnosis of adenovirus infection Klin. Lab. Diagn. 62 230-235
[8]  
Rautonen J.(2014)Peculiarities of the etiological structure of SARS in certain age and occupational groups of the population of St. Petersburg during the epidemic season of 2013–2014 Zh. Infektol. 6 62-70
[9]  
Koskiniemi M.(2015)Severe virus-associated pneumonia in military personnel, Med. Akad. 1 146-152
[10]  
Vaheri A.(2014)Detection of nonhemagglutinating influenza A(H3) viruses by enzyme-linked immunosorbent assay in quantitative influenza virus culture J. Clin. Microbiol. 52 1672-1677
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