Effects of ATP-sensitive potassium channels on the expression of P21, P27 and leptin

被引：0

作者：

Yaohui Wang

Haiyan Zheng

Shengyuan Liu

机构：

[1] Zunyi Medical College,Department of Pathophysiology

[2] Huazhong University of Science and Technology,Department of Pathophysiology, Tongji Medical College

[3] Henan University of Science and Technology,Department of Pathology and Pathophysiology, Medical College

来源：

Journal of Huazhong University of Science and Technology [Medical Sciences] | 2009年 / 29卷

关键词：

potassium channels; adipocyte; cell proliferation; cell differentiation;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

This study investigated the effects of ATP-sensitive potassium channels on the expression of P21, P27 and leptin. The expression of receptor of ATP-sensitive potassium channels (sulphonylurea receptor, SUR) mRNA in the preadipocytes and leptin mRNA was detected by PCR after rat preadipocytes were treated with the opener (diazoxide) or inhibitor (glibenclamide) of ATP-sensitive potassium channels during the process of inducing differentiation. The expression of P21 and P27 in preadipocytes treated with diazoxide or glibenclamide was assayed by Western blot. The results showed that the expression of SUR2, not SUR1 was detected in adipose tissue, preadipocytes and adipocytes. After treatment of preadipocytes with diazoxide, the expression levels of P21 and P27 were obviously higher than those in control group, but the expression levels of P21 and P27 in glibenclamide-treated group were lower than those in control group. During the process of inducing differentiation, the expression of leptin mRNA in preadipocytes treated with diazoxide was increased greatly, but the expression of leptin mRNA in glibenclamide-treated group decreased obviously. It was concluded that ATP-sensitive potassium channels might be involved in the proliferation and differentiation of rat preadipocytes by changing the expression of P21, P27 and leptin.

引用

页码：8 / 11

页数：3

共 34 条

[1]

Avram M.M.(2007)Subcutaneous fat in normal and diseased States: 3. Adipogenesis: from stem cell to fat cell JAAD 56 472-492

[2]

Avram A.S.(2007)Involvement of ATP-sensitive potassium channels in proliferation and differentiation of rat preadipocytes Acta Physiologica Sinica 59 8-12

[3]

James W.D.(1997)Expression of the cyclin dependent kinase inhibitor P21WAF1/CIP1 in human esophageal squamous cell carcinomas Virchows Arch 430 389-395

[4]

Wang Y.H.(1994)p27Kip1, a cyclin-Cdk inhibitor, links transforming growth factor-β and contact inhibition to cell cycle arrest Genes 8 9-22

[5]

Zheng H.Y.(2000)Lepin: a multifunctional hormone Cell Res 10 81-92

[6]

Qin N.L.(1987)Development of a chemically defined serum-free medium for differentiation of flat adipose precursor cells Exp Cell Res 168 15-30

[7]

Ohashi K.(2005)Effect of compound poria prescription on proliferation and differentiation and expression of PPAR-γ mRNA in rat preadipocytes Chin J Pathophysiol (Chinese) 21 1364-1367

[8]

Nemoto T.(1998)Towards understanding the assembly and structure of K-ATP channels Physiol Rev 78 227-245

[9]

Eishi Y.(1999)Role of the sulfonylurea receptor in regulating human adipocyte metabolism FASEB J 13 1833-1838

[10]

Polyak K.(2000)Partial genome scale analysis of gene expression in human adipose tissue using DNA array Obes Res 8 374-384

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