Application of quantitative PCR method in detection of Lymphocystis disease virus China (LCDV-cn) in Japanese flounder (Paralichthys olivaceus)

被引：9
作者：
Zan J. [1 ]
Sun X. [1 ]
Zhang Z. [1 ]
Qu L. [1 ]
Zhang J. [1 ]
机构：
[1] First institute of Oceanography, SOA
来源：
Chinese Journal of Oceanology and Limnology | 2007年 / 25卷 / 4期
关键词：
Lymphocystis Disease; Quantitative PCR; Virus Amount;
D O I：
10.1007/s00343-007-0418-9
中图分类号：
学科分类号：
摘要：
Lymphocystis disease causes serious economic losses in the fish farming industry. The causative agent of the disease is Lymphocystis disease virus China (LCDV-cn), which has a wide range of hosts. Based on competitive quantitative PCR technology, we established a method to quantify the LCDV-cn in tissue. Results demonstrate that the average amount of LCDV-cn in the peripheral blood of infected flounder with evident tumors is about 106 virions/ml while the average amount in those flounder with no evident tumor but cultured with the flounder with evident tumor is about 104 virions/ml. No virus was found in the negative samples of flounder. © 2007 Science Press.
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页码：418 / 422
页数：4
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