Determination of furazolidone in urine by square-wave voltammetric method

A fast, simple and sensitive square-wave voltammetric (SWV) method for the determination of trace amounts of furazolidone (FZ) in urine is reported. A three-electrode system containing stationary mercury dropping (SMDE) working electrode, Pt auxiliary electrode and Ag/AgCl reference electrode was used throughout. Briton-Rabinson buffer solution is used as both pH adjusting agent and supporting electrolyte. The calibration graph showed good linearity in the concentration range of 20–900 ng ml−1 of furazolidone with a regression coefficient of 0.9996. The equation Δ(i) = 0.0095CFZ + 0.234 was used for calculation of furazolidone concentration in the sample solution, where CFZ is the concentration of furazolidone in ng ml−1 and Δ(i) is the difference between voltammogram peak currents of sample and blank solution. The RSD for 8 replicate measurements of a 60 ng ml−1 solution and LOD of the proposed method were found to be 2.2% and 5.2 ng ml−1, respectively. The procedure was successfully applied to the determination of furazolidone in urine samples.