Fluorescent Methods in the Study of UV-Induced Changes in Structural and Functional State of Human Blood Lymphocytes

被引：0
|
作者：
V. G. Artyukhov
O. V. Putintseva
V. A. Vdovina
M. V. Pashkov
D. V. Vasilenko
机构：
[1] Voronezh State University,
[2] N. N. Burdenko Voronezh State Medical Academy,undefined
来源：
Bulletin of Experimental Biology and Medicine | 2012年 / 153卷
关键词：
fluorescent methods; lumphocytes; UV radiation; antigen-recognizing complex molecules (CD3, CD4, and CD8 markers);
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Structural and functional state of human blood lymphocytes after exposure to UV light (240-390 nm) in doses of 151-1359 J/m2 was studied by methods of laser flow cytofluorometry, indirect immunofluorescence, and fluorescent probes. Using a combination of these methods, we have showed that UV light in the specified doses induced changes in the surface phenotype of T cells: stimulation or suppression of the expression of antigen-recognizing receptor complex molecules (CD3, CD4, and CD8 markers) and their redistribution on the surface of immunocompetent cells (capping effect) with the formation of receptor clusters of various types.
引用
收藏
页码：908 / 912
页数：4
相关论文
共 5 条
  • [1] Fluorescent Methods in the Study of UV-Induced Changes in Structural and Functional State of Human Blood Lymphocytes
    Artyukhov, V. G.
    Putintseva, O. V.
    Vdovina, V. A.
    Pashkov, M. V.
    Vasilenko, D. V.
    BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE, 2012, 153 (06) : 908 - 912
  • [2] Antioxidant micronutrients improve intrinsic and UV-induced apoptosis of human lymphocytes particularly in elderly people
    A. G. Ma
    S. Ge
    M. Zhang
    X. X. Shi
    E. G. Schouten
    F. J. Kok
    Y. Y. Sun
    X. X. Han
    The journal of nutrition, health & aging, 2011, 15 : 912 - 917
  • [3] ANTIOXIDANT MICRONUTRIENTS IMPROVE INTRINSIC AND UV-INDUCED APOPTOSIS OF HUMAN LYMPHOCYTES PARTICULARLY IN ELDERLY PEOPLE
    Ma, A. G.
    Ge, S.
    Zhang, M.
    Shi, X. X.
    Schouten, E. G.
    Kok, F. J.
    Sun, Y. Y.
    Han, X. X.
    JOURNAL OF NUTRITION HEALTH & AGING, 2011, 15 (10) : 912 - 917
  • [4] Exogenous Antioxidants Impact on UV-Induced Changes in Membrane Phospholipids and the Effectiveness of the Endocannabinoid System in Human Skin Cells
    Gegotek, Agnieszka
    Jastrzab, Anna
    Dobrzynska, Marta
    Biernacki, Michal
    Skrzydlewska, Elzbieta
    ANTIOXIDANTS, 2021, 10 (08)
  • [5] The Effect of Sea Buckthorn (Hippophae rhamnoides L.) Seed Oil on UV-Induced Changes in Lipid Metabolism of Human Skin Cells
    Gegotek, Agnieszka
    Jastrzab, Anna
    Jarocka-Karpowicz, Iwona
    Muszynska, Marta
    Skrzydlewska, Elzbieta
    ANTIOXIDANTS, 2018, 7 (09)
1