In this study, the effect of H2O2-induced oxidative stress on gellan gum production and cell growth were investigated. Gellan gum production was improved and cell growth was inhibited by H2O2. A multiple H2O2 stresses with different concentrations were developed to optimize gellan gum production. A maximal gellan gum yield (22.52 g/L), which was 35.58 % higher than the control, was observed with 2, 2, 3, 4 mmol/L H2O2 added at 6, 12, 18, 24 h, respectively. Moreover, UDP-glucose pyrophosphorylase activity and glucosyltransferase activity were increased with H2O2 stresses. This new strategy of multiple H2O2-induced oxidative stresses would be further applied to gellan gum production in future study.
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Univ Southampton, Fac Engn & Phys Sci, Electrochem Engn Lab, Southampton SO17 1BJ, Hants, England
Univ Southampton, Fac Engn & Phys Sci, nCATS, Southampton SO17 1BJ, Hants, EnglandUniv Southampton, Fac Engn & Phys Sci, Electrochem Engn Lab, Southampton SO17 1BJ, Hants, England
Mavrikis, Sotirios
Goeltz, Maximilian
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Friedrich Alexander Univ Erlangen Nurnberg, Fac Engn, Mat Sci & Engn Met, D-91058 Erlangen, GermanyUniv Southampton, Fac Engn & Phys Sci, Electrochem Engn Lab, Southampton SO17 1BJ, Hants, England
Goeltz, Maximilian
Rosiwal, Stefan
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Friedrich Alexander Univ Erlangen Nurnberg, Fac Engn, Mat Sci & Engn Met, D-91058 Erlangen, GermanyUniv Southampton, Fac Engn & Phys Sci, Electrochem Engn Lab, Southampton SO17 1BJ, Hants, England
Rosiwal, Stefan
Wang, Ling
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Univ Southampton, Fac Engn & Phys Sci, nCATS, Southampton SO17 1BJ, Hants, EnglandUniv Southampton, Fac Engn & Phys Sci, Electrochem Engn Lab, Southampton SO17 1BJ, Hants, England
Wang, Ling
Ponce de Leon, Carlos
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Univ Southampton, Fac Engn & Phys Sci, Electrochem Engn Lab, Southampton SO17 1BJ, Hants, England
Univ Southampton, Fac Engn & Phys Sci, nCATS, Southampton SO17 1BJ, Hants, EnglandUniv Southampton, Fac Engn & Phys Sci, Electrochem Engn Lab, Southampton SO17 1BJ, Hants, England