13C metabolite profiling to compare the central metabolic flux in two yeast strains

被引:0
|
作者
Joon-Young Jung
Seok Hun Yun
Dae-Kyun Im
Jinwon Lee
Min-Kyu Oh
机构
[1] Korea University,Department of Chemical and Biological Engineering
[2] Sogang University,Department of Chemical and Biomolecular Engineering
[3] CJ Research Institute of Biotechnology,undefined
来源
Biotechnology and Bioprocess Engineering | 2016年 / 21卷
关键词
C metabolite profiling; stimulus response experiments; metabolic flux analysis; yeast;
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学科分类号
摘要
13C metabolite profiling to quantify the dynamic changes of central carbon metabolites was attempted using mass isotopomer distribution analysis in two yeast strains, Saccharomyces cerevisiae and Kluyveromyces marxianus. Mass and isotopomer balances of the intermediates were examined and calculated in both yeast species and central carbon metabolic fluxes were successfully determined. Metabolic fluxes of pentose phosphate pathway in K. marxianus were 1.66 times higher than S. cerevisiae. The flux difference was also supported by relatively high abundance of partially labeled fructose 6-phosphate and 3-phosphoglycerate as well as an increased concentration of labeled L-valine in K. marxianus. Metabolic flux analysis combined with dynamic metabolite profiling has provided better understanding in the central carbon metabolic pathways of two model organisms and can be applied as a method to analyze more complicated metabolic networks in other organisms.
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页码:814 / 822
页数:8
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