Involvement of IL-2/IL-2R system activation by parasite antigen in polyclonal expansion of CD4+25+ HTLV-1-infected T-cells in human carriers of both HTLV-1 and S. stercoralis

被引：0

作者：

Masao Satoh

Hiromu Toma

Kazuyuki Sugahara

Ken-ichiro Etoh

Yoshiyuki Shiroma

Susumu Kiyuna

Masahiro Takara

Masao Matsuoka

Kazunari Yamaguchi

Kiyonobu Nakada

Koichiro Fujita

Somei Kojima

Eitaro Hori

Yuetsu Tanaka

Shimeru Kamihira

Yoshiya Sato

Toshiki Watanabe

机构：

[1] Institute of Medical Science,Division of Pathology

[2] University of Tokyo,Department of Medical Zoology

[3] Tokyo,Department of Parasitology

[4] Saitama Medical School,Department of Laboratory Medicine

[5] Saitama,Department of Internal Medicine

[6] School of Medicine,Department of Medical Zoology

[7] University of the Ryukyu,Department of Parasitology

[8] Nagasaki University School of Medicine,Department of Infectious Disease and Immunology

[9] Kumamoto University School of Medicine,undefined

[10] Kumamoto,undefined

[11] Izumizaki Hospital,undefined

[12] Gushikawa Clinic,undefined

[13] Institute for Virus Research,undefined

[14] Kyoto University,undefined

[15] Blood Transfusion Service,undefined

[16] School of Medicine,undefined

[17] Kumamoto University,undefined

[18] Okinawa Kyodo Hospital,undefined

[19] Faculty of Medicine,undefined

[20] Tokyo Medical and Dental University,undefined

[21] Institute of Medical Science,undefined

[22] University of Tokyo,undefined

[23] Okinawa-Asia Research Center of Medical Science,undefined

[24] Faculty of Medicine,undefined

[25] University of the Ryukyus,undefined

来源：

Oncogene | 2002年 / 21卷

关键词：

HTLV-1 provirus load; clonality; antigen; IL-2; ATL;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

The intermediate state of HTLV-1 infection, often found in individuals dually infected with Strongyloides stercoralis (S. stercoralis) and HTLV-1, is assumed to be a preleukemic state of adult T-cell leukemia (ATL). To investigate the effects of S. stercoralis superinfection on the natural history of HTLV-1 infection, we characterized peripheral blood samples of these individuals in Okinawa, Japan, an endemic area for both HTLV-1 and S. stercoralis and we studied effects of the parasite antigen on T-cells. The dually infected individuals showed a significantly higher provirus load and an increase in CD4+25+ T cell population, with a significant, positive correlation. This increase was attributable to polyclonal expansion of HTLV-1-infected cells, as demonstrated by inverse-long PCR analysis of the integration sites. S. stercoralis antigen activated the IL-2 promoter in reporter gene assays, induced production of IL-2 by PBMC in vitro, and supported growth of IL-2 dependent cell lines immortalized by HTLV-1 infection or the transduction of Tax. Taken collectively, these results indicate that S. stercoralis infection induces polyclonal expansion of HTLV-1-infected cells by activating the IL-2/IL-2R system in dually infected carriers, an event which may be a precipitating factor for ATL and inflammatory diseases.

引用

页码：2466 / 2475

页数：9