Thermal characterisation of actin filaments prepared from adp-actin monomers

被引：0
|
作者：
J. Orbán
Kinga Pozsonyi
Krisztina Szarka
Szilvia Barkó
Emőke Bódis
D. Lőrinczy
机构：
[1] University of Pécs,Faculty of Medicine, Department of Biophysics
[2] Office for Academy Research Groups Attached to Universities and other Institutions at University of Pécs,Research Group for Fluorescence Spectroscopy
来源：
Journal of Thermal Analysis and Calorimetry | 2006年 / 84卷
关键词：
actin; denaturation; DSC; nucleotides; thermodynamics;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The thermodynamic properties of the ADP- and ATP-actin filaments were compared by the method of differential scanning calorimetry. The lower melting point for the ADP-F-actin filament (58.4 vs. 64.5°C for ATP-F-actin) indicated that compared to the ATP-actin filaments its structure was less resistant to heat denaturation. The detailed thermodynamic characterisation of the proteins was carried out by the analysis of the calorimetric enthalpy, the entropy and the free enthalpy changes. All of the determined parameters gave lower values to the ADP-actin filaments than to the ATP-actin filaments. The calculated values of the activation energy also demonstrated that compared to the ADP-F-actin the ATP-F-actin was thermodynamically more resistant to the denaturing effect of heat.
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页码：619 / 623
页数：4
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