Fungal communities from methane hydrate-bearing deep-sea marine sediments in South China Sea

被引：0
作者：
Xintian Lai
Lixiang Cao
Hongming Tan
Shu Fang
Yali Huang
Shining Zhou
机构：
[1] College of Life Sciences,State Key Laboratory for Biocontrol and Department of Biochemistry
[2] Zhongshan (Sun Yatsen) University,undefined
[3] 3Current address: Shenzhen Academy of Metrology and Quality Inspection.,undefined
来源：
The ISME Journal | 2007年 / 1卷
关键词：
methane hydrate; deep-sea marine sediments; fungal diversity; internal transcribed spacer (ITS); South China Sea;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
To elucidate fungal diversity in methane hydrate-bearing deep-sea marine sediments in the South China Sea, internal transcribed spacer (ITS) regions of rRNA genes from five different sediment DNA samples were amplified and phylogenetically analyzed. Total five ITS libraries were constructed and 413 clones selected randomly were grouped into 24 restriction patterns by Amplified Ribosomal DNA Restriction Analysis (ARDRA). ITS sequences of 44 representative clones were determined and compared with the GenBank database using gapped-BLAST. The phylogenetic analysis showed that the ITS sequences (71–97% similarity) were similar to those of Phoma, Lodderomyces, Malassezia, Cryptococcus, Cylindrocarpon, Hortaea, Pichia, Aspergillus and Candida. The remaining sequences were not associated to any known fungi or fungal sequences in the public database. The results suggested that methane hydrate-bearing deep-sea marine sediments harbor diverse fungi. This is the first report on fungal communities from methane hydrate-bearing deep-sea marine sediments in South China Sea.
引用
收藏
页码：756 / 762
页数：6
相关论文
共 125 条
[1]  
Anderson IC(2004)Diversity and ecology of soil fungal communities: increased understanding through the application of molecular techniques Environ Microbiol 6 769-779
[2]  
Cairney JWG(2003)Diversity of fungi in organic soils under a moorland-Scots pine ( Environ Microbiol 5 1121-1132
[3]  
Anderson IC(2003) L.) gradient Environ Microbiol 5 36-47
[4]  
Campbell CD(1997)Potential bias of fungal 18S rDNA and internal transcribed spacer polymerase chain reaction primers for estimating fungal biodiversity in soil Nucleic Acids Res 25 3389-3402
[5]  
Prosser JI(1975)Gapped-BLAST and PSI-BLAST: a new generation of protein database search programs Appl Microbiol 30 309-318
[6]  
Anderson IC(2002)Survival of human enteric and other sewage microorganisms under simulated deep-sea conditions Microb Ecol 43 329-340
[7]  
Campbell CD(2002)Analysis of internal transcribed spacer (ITS) regions of rRNA genes in fungal communities in a southeastern US salt marsh Mycol Res 106 532-540
[8]  
Prosser JI(2003)Investigation of the influence of prescribed burning on ITS profiles of ectomycorrhizal and other soil fungi at three Australian sclerophyll forest sites FEMS Microbiol Ecol 44 153-163
[9]  
Altschul SF(1993)Efficient recovery of environmental DNA for expression cloning by indirect extraction methods Mol Ecol 2 113-118
[10]  
Madden TL(2000)ITS primers with enhanced specificity for basidiomycetes: application to the identification of mycorrhiza and rusts Appl Microbiol Biotechnol 54 741-750
12345678910