Primary hepatocytes and their cultures in liver apoptosis research

被引：0
作者：
Mathieu Vinken
Michaël Maes
André G. Oliveira
Bruno Cogliati
Pedro E. Marques
Gustavo B. Menezes
Maria Lúcia Zaidan Dagli
Tamara Vanhaecke
Vera Rogiers
机构：
[1] Vrije Universiteit Brussel (VUB),Department of Toxicology (FAFY), Faculty of Medicine and Pharmacy, Center for Pharmaceutical Research (CePhaR)
[2] Universidade Federal de Minas Gerais (UFMG),Department of Morphology, Institute of Biological Sciences
[3] University of São Paulo,Department of Pathology, School of Veterinary Medicine and Animal Science
来源：
Archives of Toxicology | 2014年 / 88卷
关键词：
Cell death; Apoptosis; Primary hepatocyte; In vitro culture; Assays; Biomarkers;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Apoptosis not only plays a key role in physiological demise of defunct hepatocytes, but is also associated with a plethora of acute and chronic liver diseases as well as with hepatotoxicity. The present paper focuses on the modelling of this mode of programmed cell death in primary hepatocyte cultures. Particular attention is paid to the activation of spontaneous apoptosis during the isolation of hepatocytes from the liver, its progressive manifestation upon the subsequent establishment of cell cultures and simultaneously to strategies to counteract this deleterious process. In addition, currently applied approaches to experimentally induce controlled apoptosis in this in vitro setting for mechanistic research purposes and thereby its detection using relevant biomarkers are reviewed.
引用
收藏
页码：199 / 212
页数：13
相关论文
共 812 条
  • [1] Abou-Elella AM(2002)Tumour necrosis factor-alpha and nitric oxide mediate apoptosis by Can J Gastroenterol 16 791-799
  • [2] Siendones E(1994)-galactosamine in a primary culture of rat hepatocytes: exacerbation of cell death by cocultured Kupffer cells Hepatology 20 494-514
  • [3] Padillo J(2009)Recent advances in the isolation of liver cells Biomaterials 30 26-34
  • [4] Montero JL(1995)The compatibility of hepatocytes with chemically modified porous silicon with reference to in vitro biosensors Hepatology 22 175-187
  • [5] De la Mata M(2010)A morphological study of differentiated hepatocytes in vitro J Virol 84 11994-12001
  • [6] Muntane Relat J(2006)Apoptosis of hepatitis B virus-infected hepatocytes prevents release of infectious virus Hepatogastroenterology 53 747-752
  • [7] Alpini G(2001)Hepatocyte proliferation and apoptosis in rat liver after liver injury Cell Death Differ 8 279-288
  • [8] Phillips JO(2002)Dexamethasone inhibits spontaneous apoptosis in primary cultures of human and rat hepatocytes via Bcl-2 and Bcl-xL induction Cell Death Differ 9 945-955
  • [9] Vroman B(2011)Spontaneous apoptosis in primary cultures of human and rat hepatocytes: molecular mechanisms and regulation by dexamethasone Cell Mol Biol Lett 16 328-341
  • [10] LaRusso NF(1997)DMSO modulates the pathway of apoptosis triggering J Pathol 183 177-181
12345678910