An assessment of somaclonal variation in micropropagated plants of sugarcane by RAPD markers

被引：21

作者：

Tawar P.N. ^{[1
]}

Sawant R.A. ^{[1
]}

Dalvi S.G. ^{[1
]}

Nikam A.A. ^{[1
]}

Kawar P.G. ^{[2
]}

Devarumath R.M. ^{[2
]}

机构：

[1] Tissue Culture Section, Vasantdada Sugar Institute, Manjari

[2] Molecular Biology and Genetic Engineering Laboratory, Vasantdada Sugar Institute, Manjari

来源：

Sugar Tech | 2008年 / 10卷 / 2期

关键词：

Micropropagation; RAPD; Somaclonal variation; Sugarcane;

D O I：

10.1007/s12355-008-0021-1

中图分类号：

学科分类号：

摘要：

Sugarcane variety Co 94012 is a new variety released in India through the use of somaclonal variation. Significant differences were found in quantitative and qualitative agronomic characters. Another somaclone VSI 434 is an extra early maturing variety with significant difference in morphological and qualitative characters over the parent CoC 671. The genetic variation of micropropagated plantlets was tested by RAPD analysis. The banding pattern of PCR amplified products from micropropagated plantlets showed that most of them were monomorphic in both the varieties. The amplicon pattern of VSI 434 and Co 94012 with primers OPA 17 and OPA 19 differed from parent CoC 671, respectively. But with other primers they were more or less monomorphic indicating that the amplicon from these two primers can be used as potential fingerprint for Co VSI 434 and Co 94012. © 2008 Society for Sugar Research & Promotion.

引用

页码：124 / 127

页数：3

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