The 3D-Structural Modeling of Yeast D-Amino Acid Oxidase

被引：1

作者：

Tishkov V.I. ^{[1
,2
,3
]}

Khoronenkova S.V. ^{[1
,2
]}

Cherskova N.V. ^{[2
,3
]}

Savin S.S. ^{[2
,3
]}

Uporov I.V. ^{[1
]}

机构：

[1] Division of Chemical Enzymology, Department of Chemistry, Moscow State University

[2] Innovations and High Technology Department, Moscow State University

[3] A.B. Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow 119071, Leninskii pr.

来源：

Moscow University Chemistry Bulletin | 2010年 / 65卷 / 3期

关键词：

computer modeling; D-amino acid oxidase; protein engineering; structure; Trigonopsis variabilis;

D O I：

10.3103/S0027131410030028

中图分类号：

学科分类号：

摘要：

D-amino acid oxidase from the yeast Trigonopsis variabilis (TvDAAO) is used in the pharmaceutical industry and fine organic synthesis but for future practical applications new mutant forms of the enzyme with improved stability and catalytic properties are needed. Experiments on the crystallization of TvDAAO have been carried out for the last three decades without any success. For protein engineering of the enzyme using a rational design approach a model 3D-structure of TvDAAO was built using the homology modeling method. Cys108 and Cys298 residues were proposed for site-directed mutagenesis after analysis of the enzyme model structure. © 2010 Allerton Press, Inc.

引用

页码：121 / 126

页数：5

共 18 条

[1]

Pollegioni L., Piubelli L., Sacchi S., Pilone M.S., Molla G., Cell Mol. Life Sci., 64, (2007)

[2]

Khoronenkova S.V., Tishkov V.I., Biochemistry (Moscow), 73, (2008)

[3]

Khoronenkova S.V., Tishkov V.I., Biochemistry, 70, (2005)

[4]

Pollegioni L., Molla G., Sacchi S., Rosini E., Verga R., Pilone M.S., Appl. Microbiol. Biotechnol., 78, (2008)

[5]

Davydova E.E., Tishkov V.I., Moscow Univ. Chem. Bulletin, 43, (2002)

[6]

Umhau S., Pollegioni L., Molla G., Diederichs K., Welte W., Pilone M.S., Ghisla S., Proc. Natl. Acad. Sci. USA., 97, (2000)

[7]

Mizutani H., Miyahara I., Hirotsu K., Nishina Y., Shiga K., Setoyama C., Miura R., J. Biochem. (Tokyo), 120, (1996)

[8]

Mattevi A., Vanoni M.A., Todone F., Rizzi M., Teplyakov A., Coda A., Bolognesi M., Curti B., Proc. Natl. Acad. Sci. USA., 93, (1996)

[9]

Miura R., Setoyama C., Nishina Y., Shiga K., Mizutani H., Miyahara I., Hirotsu K., J. Biochem. (Tokyo), 122, (1997)

[10]

Todone F., Vanoni M.A., Mozzarelli A., Bolognesi M., Coda A., Curti B., Mattevi A., Biochemistry, 36, (1997)

← 1 2 →