Phytochemical screening, antimicrobial, antioxidant and cytotoxic potential of different extracts of Psidium guajava leaves

被引：12

作者：

Raj A. ^{[1
]}

Menon V. ^{[1
]}

Sharma N. ^{[1
]}

机构：

[1] Department of Biotechnology, Chandigarh Group of Colleges, Landran, Mohali, Punjab

来源：

Vegetos | 2020年 / 33卷 / 4期

关键词：

Antimicrobial; Antioxidant; Cytotoxicity; Phytochemicals; Psidium gua[!text type='java']java[!/text; Solvent;

D O I：

10.1007/s42535-020-00151-4

中图分类号：

学科分类号：

摘要：

Psidium guajava (Myrtaceae) is an important food crop and medicinal plant supporting its traditional uses. The aim of the present study was to evaluate phytochemical, antimicrobial, antioxidant and cytotoxic activity of various solvent extracts of leaves of P. guajava. Phytochemical analysis revealed the presence of terpenoids, tannins, steroids, flavonoids, saponins, alkaloids and highest amount of phytocompounds was reported with methanol, ethanol and acetone extract. Total phenolic and flavonoid content was found to be highest in acetone extract (256.32 ± 4.56 mg/g GAE) and n-butanol extract (198.64 ± 1.23 mg/g RE) respectively. The antimicrobial assay revealed that acetone extract (13.5 ± 2.12 mm) exhibit maximum zone of inhibition against P. aeruginosa; while acetone (14 ± 0.71 mm) and ethanol (14.5 ± 2.12 mm) showed maximum zone of inhibition against S. aureus. Chloroform extract (10.2 ± 1.13 mm) showed maximum inhibition to the growth of C. albicans. Guava leaves also possess antioxidant activity which was evaluated in terms of % DPPH (2, 2-diphenyl-1-picrylhydrazyl) free radical scavenging activity and was found highest in acetone extract (41%). Cytotoxic activity was evaluated in terms of stimulation index using bovine lymphocytes and highest stimulation index was found with methanol extract (1.432 ± 0.193). This study revealed that acetone extract of guava leaf comprises effective antimicrobial, antioxidant and cytotoxicity and can be used for therapeutic applications. © 2020, Society for Plant Research.

引用

页码：750 / 758

页数：8

共 58 条

[1]

Amella M., Bronner C., Briancon F., Haag M., Anton R., Landry Y., Inhibition of mast cell histamine release by flavonoids and bioflavonoids, Planta Med, 51, pp. 16-20, (1985)

[2]

Arima H., Danno G., Isolation of antimicrobial compounds from Guava (Psidium guajava L.) and their structural elucidation, Biosci Biotechnol Biochem, 66, pp. 1727-1730, (2002)

[3]

Ashraf A., Sarfraz R.A., Rashid M.A., Mahmood A., Shahid M., Noor N., Chemical composition, antioxidant, antitumor, anticancer and cytotoxic effects of Psidium guajava leaf extracts, Pharmaceut Biol, 54, 10, pp. 1971-1981, (2016)

[4]

Bae H., Jayaprakasha G.K., Crosby K., Jifon J.L., Patil B.S., Influence of extraction solvents on antioxidant activity and the content of bioactive compounds in non-pungent peppers, Plant Foods Hum Nutr, 67, pp. 120-128, (2012)

[5]

Barbalho S.M., Farinazzi-Machado F.M., de Alvares G.R., Brunnati A.C., Otoboni A.M., Ottoboni B., Psidium guajava (Guava): a plant of multipurpose medicinal applications, Med Aromat Plants, 1, 104, pp. 2167-2412, (2012)

[6]

Barbour E.K., Al Sharif M., Sagherian V.K., Habre A.N., Talhouk R.S., Talhouk S.N., Screening of selected indigenous plants of Lebanon for antimicrobial activity, J Ethnopharmacol, 93, 1, pp. 1-7, (2004)

[7]

Barros L., Ferreira M.J., Queiros B., Ferreira I.C.F.R., Baptista P., Total phenols, ascorbic acid, β-carotene and lycopene in Portuguese wild edible mushrooms and their antioxidant activities, Food Chem, 103, pp. 413-419, (2007)

[8]

Biswas B., Rogers K., McLaughlin F., Daniels D., Yadav A., Antimicrobial activities of leaf extracts of guava (Psidium guajava L.) on two gram-negative and gram-positive bacteria, Int J Microbiol, (2013)

[9]

Camarena-Tello J.C., Martinez-Flores H.E., Garnica-Romo M., Padilla-Ramirez J.S., Saavedra-Molina A., Alvarez-Cortes O., Bartolome-Camacho M.C., Rodiles-Lopez J.O., Quantification of phenolic compounds and in vitro radical scavenging abilities with leaf extracts from two varieties of Psidium guajava L, Antioxidants, 7, 3, (2018)

[10]

Chaundry P.S., Cabrera J., Juliana H.R., Varma S.D., Inhibition of human lens aldose reductase by flavonoids, sulindac and indomethacin, Biochem Pharmacol, 2, pp. 1995-1998, (1983)

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