A vertebrate case study of the quality of assemblies derived from next-generation sequences

被引：0
|
作者：
Liang Ye
LaDeana W Hillier
Patrick Minx
Nay Thane
Devin P Locke
John C Martin
Lei Chen
Makedonka Mitreva
Jason R Miller
Kevin V Haub
David J Dooling
Elaine R Mardis
Richard K Wilson
George M Weinstock
Wesley C Warren
机构：
[1] The Genome Center,
[2] Washington University School of Medicine,undefined
[3] The J Craig Venter Institute,undefined
来源：
Genome Biology | / 12卷
关键词：
Segmental Duplication; Chicken Genome; Test Assembly; Single Base Substitution; Assembly Quality;
D O I：
暂无
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学科分类号：
摘要：
The unparalleled efficiency of next-generation sequencing (NGS) has prompted widespread adoption, but significant problems remain in the use of NGS data for whole genome assembly. We explore the advantages and disadvantages of chicken genome assemblies generated using a variety of sequencing and assembly methodologies. NGS assemblies are equivalent in some ways to a Sanger-based assembly yet deficient in others. Nonetheless, these assemblies are sufficient for the identification of the majority of genes and can reveal novel sequences when compared to existing assembly references.
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