Genome-wide analysis of fluoride exporter genes in plants

被引：0
作者：
Samridhi Agarwal
Preetom Regon
Mehzabin Rehman
Bhaben Tanti
Sanjib Kumar Panda
机构：
[1] Central University of Rajasthan,Department of Biochemistry
[2] Assam University,Department of Life Science and Bioinformatics
[3] Gauhati University,Department of Botany
来源：
3 Biotech | 2021年 / 11卷
关键词：
Fluoride toxicity; FEX; Gene expression; Plants;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Fluoride exporter genes (FEX) are known for the expulsion of cytoplasmic fluoride, thus preventing fluoride toxicity in plants. In this study, 31 FEX genes were identified across 19 plant species. Camphor Resistance (CrcB) domain was found to be present in all the identified FEX genes in plants. FEX genes were sequentially very conserved among the plants and are located mostly in chloroplast and mitochondria. The tertiary structure (3D) of AtFEX1 suggests that FEX genes of plants possess pore I and pore II, necessary for fluoride export. The TTFSGWNQ and GCLSTVSTF motifs were found to be well conserved in pore I and pore II. Phenylalanine (Phe/F) was also present in both the motifs, necessary for fluoride ions recognition and export. Cis-acting analysis in promoter sequences of plant FEX revealed several elements associated with various functions such as phytohormone signaling, integrating biotic and abiotic stress responses in plants. Prolong fluoride exposure causes necrosis in young leaves in Vigna radiata. Expression of VrFEX1 and VrFEX2 were highly induced under exogenous fluoride, thus suggesting a possible role in fluoride detoxification.
引用
收藏
相关论文
共 144 条
[1]  
Altschul SF(1990)Basic local alignment search tool J MolBiol 215 403-410
[2]  
Gish W(2019)Detecting sequence signals in targeting peptides using deep learning Life Sci Alliance 2 1-14
[3]  
Miller W(2009)MEME Suite: tools for motif discovery and searching Nucleic Acids Res 37 202-208
[4]  
Armenteros JJA(2012)Widespread genetic switches and toxicity resistance proteins for fluoride Science 335 233-235
[5]  
Salvatore M(2019)Structural introspection of a putative fluoride transporter in plants Biotech 9 103-485
[6]  
Emanuelsson O(2015)The arabidopsis information resource: making and mining the “gold standard” annotated reference plant genome Genesis 53 474-20
[7]  
Bailey TL(2017)Fluoride export (FEX) proteins from fungi, plants and animals are “single barreled” channels containing one functional and one vestigial ion pore PLoS ONE 12 1-43
[8]  
Boden M(2010)Effect of sodium fluoride on seed g ermination and seedling growth of triticumaestivum VAR. RAJ. 4083 J Phytol 2010 41-140
[9]  
Buske FA(2016)Ensembl plants: integrating tools for visualizing, mining, and analyzing plant genomics data Methods MolBiol 1374 115-262
[10]  
Baker JL(2015)Biochemical and antioxidant responses of paddy ( Fluoride 48 56-D1163
12345678910