Endocannabinoid metabolomics: A novel liquid chromatography-mass spectrometry reagent for fatty acid analysis

被引：0

作者：

John Williams

Lakshmipathi Pandarinathan

Jodi Anne Wood

Paul Vouros

Alexandros Makriyannis

机构：

[1] Northeastern University,Center for Drug Discovery

[2] Northeastern University,Barnett Institute and Department of Chemistry

来源：

The AAPS Journal | / 8卷

关键词：

fatty acids; LC-MS; metabolome; oxazoline derivatives; microwave synthesis;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

We have synthesized 4,4-dimethoxyoxazoline derivatives of several fatty acids associated with the endocannabinoid metabolome using tris(hydroxymethyl)aminomethane in a 1-step reaction by microwave irradiation. The derivatization incorporates a nitrogen into the final product, which allows for improved detection by liquid chromatographymass spectrometry in positive atmospheric pressure chemical ionization (APCI) mode. Palmitic and oleic acid derivatives show a 200-fold increase in sensitivity compared with the free acids when analyzed in negative-mode APCI. In addition to improving sensitivity, the oxazoline derivatization creates a similar ionization response for the fatty acids tested, which simplifies their quantitation. Fatty acid oxazoline derivatives can be detected using the same conditions optimized for the endocannabinoids, which allows for a simultaneous quantitation of the entire endocannabinoid metabolome.

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