Distance and dynamics determination by W-band DEER and W-band ST-EPR

被引：0
作者：
Likai Song
Mioara Larion
Jean Chamoun
Marco Bonora
Piotr G. Fajer
机构：
[1] Florida State University,National High Magnetic Field Laboratory, Institute of Molecular Biophysics, Department of Biological Science, Department of Biochemistry and Chemistry
来源：
European Biophysics Journal | 2010年 / 39卷
关键词：
EPR; W-band; ST-EPR; DEER;
D O I：
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中图分类号：
学科分类号：
摘要：
To explore high-field EPR in biological applications we have compared measurements of dynamics with X-band (9 GHz) and W-band (94 GHz) saturation transfer EPR (ST-EPR) and distance determination by X and W-band DEER. A fourfold increase of sensitivity was observed for W-band ST-EPR compared with X-band. The distance measurements at both fields showed very good agreement in both the average distances and in the distance distributions. Multifrequency EPR thus provides an additional experimental dimension to facilitate extraction of distance populations. However, the expected orientational selectivity of W-band DEER to determine the relative orientation of spins has not been realized, most likely because of the large orientational disorder of spin labels on the protein surface.
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页码：711 / 719
页数：8
相关论文
共 69 条
[1]  
Brown LJ(2002)Structure of the inhibitory region of troponin by site directed spin labeling electron paramagnetic resonance Proc Natl Acad Sci USA 99 12765-12770
[2]  
Sale KL(2006)High-field pulsed electron-electron double resonance spectroscopy to determine the orientation of the tyrosyl radicals in ribonucleotide reductase Proc Natl Acad Sci USA 103 13386-13390
[3]  
Hills R(1983)Sensitivity of Saturation Transfer ESR Spectra to Anisotropic Rotation. Application to Membrane Systems J Magn Reson 51 446-459
[4]  
Rouviere C(2007)Practical Pulsed Dipolar ESR (DEER) ESR Spectrosc Membr Biophy 27 95-128
[5]  
Song L(1984)Standard conditions for the measurement of saturation-transfer ESR spectra J Magn Reson 59 160-163
[6]  
Zhang X(2004)High field/high frequency saturation transfer electron paramagnetic resonance spectroscopy: increased sensitivity to very slow rotational motions Biophys J 86 3940-3950
[7]  
Fajer PG(1973)New EPR methods for the study of very slow motion: application to spin-labeled hemoglobin Ann NY Acad Sci 222 680-692
[8]  
Denysenkov VP(1982)A comparison of resolution-enhancement methods in saturation-transfer EPR. 15 N isotopically substituted spin labels and 35 GHz high-frequency operation Biophys J 37 563-567
[9]  
Prisner TF(1978)Photon correlation spectroscopy of hemoglobin: diffusion of oxy-HbA and oxy-HbS Biopolymers 17 1581-1593
[10]  
Stubbe J(1993)Double electron-electron resonance spin-echo modulation: spectroscopic measurement of electron spin pair separations in orientationally disordered solids J Chem Phys 98 5134-5146
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