Apoptosis of lymphoma cells is abolished due to blockade of cytochrome c release despite Nur77 mitochondrial targeting

被引：0
作者：
Andrzej Rapak
Izabela Stasik
Ewa Ziolo
Leon Strzadala
机构：
[1] Polish Academy of Sciences,Ludwik Hirszfeld Institute of Immunology and Experimental Therapy
来源：
Apoptosis | 2007年 / 12卷
关键词：
Apoptosis; Lymphoma; Nur77; FK506; Mitochondria; Cytochrome c;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Nur77 is reported to undergo translocation to mitochondria in response to apoptotic signaling in a variety of cancer cell lines. It was shown that on the mitochondrial membrane, Nur77 interacts with Bcl-2, leading to the conversion of this protein from a protector to a killer with subsequent release of cytochrome c to the cytosol. Here it is shown that in thymic lymphoma cells resistant to calcium-mediated apoptosis, cytochrome c release is abolished despite of Nur77 mitochondrial targeting. However, cytochrome c release and apoptosis can be restored by treatment with FK506. Hence, the molecular target regulation of the sensitivity of lymphoma cells to calcium signaling is associated with cytochrome c release and is FK506 sensitive. These results provide new insight into the role of FK506-sensitive factors as a critical link between calcium signaling and resistance of lymphoma cells to death.
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页码：1873 / 1878
页数：5
相关论文
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  • [1] He YW(2002)Orphan nuclear receptors in T lymphocyte development J Leukoc Biol 72 440-446
  • [2] Kuang AA(1999)Nur77 transcription activity correlates with its apoptotic function in vivo Eur J Immunol 29 3722-3728
  • [3] Cado D(1996)Apoptosis of nur77/N10-transgenic thymocytes involves the Fas/Fas ligand pathway Proc Natl Acad Sci USA 93 5533-5539
  • [4] Winoto A(2000)Cytochrome c release and apoptosis induced by mitochondrial targeting of nuclear orphan receptor TR3 Science 289 1159-1164
  • [5] Weih F(1998)Thymic expression of the transcription factor Nur77 rescues the T cell but not the B cell abnormality of gld/gld mice J Immunol 161 4252-4256
  • [6] Ryseck RP(1995)A role for the orphan steroid receptor Nur77 in apoptosis accompanying antigen-induced negative selection Immunity 3 273-282
  • [7] Chen L(1994)Requirement for the orphan steroid receptor Nur77 in apoptosis of T-cell hybridomas Nature 367 277-281
  • [8] Bravo R(1994)Apoptotic signals delivered through the T-cell receptor of a T-cell hybrid require the immediate-early gene nur77 Nature 367 281-284
  • [9] Li H(2003)Transcriptional activation of known and novel apoptotic pathways by Nur77 orphan steroid receptor EMBO J 22 6526-65
  • [10] Kolluri SK(2002)Nuclear hormone receptors in T lymphocytes Cell 109 S57-S66
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