Membrane Rigidity Determined by Atomic Force Microscopy Is a Parameter of the Permeability of Liposomal Membranes to the Hydrophilic Compound Calcein

被引：0
作者：
Yuki Takechi-Haraya
Kumiko Sakai-Kato
Yukihiro Goda
机构：
[1] Japan Agency for Medical Research and Development,Division of Drugs
[2] National Institute of Health Sciences,undefined
来源：
AAPS PharmSciTech | 2017年 / 18卷
关键词：
atomic force microscopy; calcein; liposomal membrane permeability; liposome; membrane rigidity;
D O I：
暂无
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学科分类号：
摘要：
We determined the permeability coefficient of a model hydrophilic drug, calcein, encapsulated within saturated lipid-based nano-sized liposomes of various lipid profiles. We demonstrated that the addition of cholesterol to liposomes containing saturated lipids increased the permeability of the liposomal membrane to calcein via a decrease in the membrane bending modulus, as determined by means of atomic force microscopy. We found an inverse correlation between the membrane bending modulus of saturated lipid-based nano-sized liposomes and the permeability coefficient of encapsulated calcein, demonstrating that bending modulus, as determined by means of atomic force microscopy, is a quantitative parameter describing the permeability of liposomal membranes to calcein.
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页码：1887 / 1893
页数：6
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