A sensitive and economical modified method for estimation of cerebrospinal fluid proteins

被引:7
作者
Durgawale P. [1 ]
Kanase S. [1 ]
Shukla P.S. [1 ,3 ]
Sontakke S. [2 ]
机构
[1] Department of Biochemistry, Krishna Institute of Medical Sciences, Karad, Maharashtra
[2] Dept. of Biochemistry, Shivaji University, Kolhapur
[3] Dept. of Biochemistry, Krishna Institute of Medical Sciences, 415 110, Maharashtra
关键词
C.S.F; Protein; Modified bromocresol green (BCG) method; Pyrogallol red method (Kit); SPSS 10.1 Statistical software;
D O I
10.1007/BF02867422
中图分类号
学科分类号
摘要
Cerebrospinal fluid protein estimation is a routine diagnostic tool for various diseases. The proposed modified bromocresol green (BCG) method is applicable to automated as well as manual measurements. Protein concentrations and absorbance at 600 nm are linearly related throughout a wide range of concentrations from 10 mg % to 2000 mg %. Ninety C.S.F. samples were analyzed and results obtained by both the modified BCG method and routine pyrogallol red method were compared. An excellent co-relation was observed (n = 90 , g = 0.996, P< 0.001).
引用
收藏
页码:174 / 177
页数:3
相关论文
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