The Aggregation of Aβ42 Induced by Nano Copper and the Antagonistic Action of Polysaccharides

被引：0
作者：
Wei Wang
Guoguang Zhang
Jinmei Zou
机构：
[1] Minnan Normal University,Department of Biological Sciences and BioTechnology
来源：
Applied Biochemistry and Biotechnology | 2015年 / 175卷
关键词：
Aβ; Aggregation; Copper nanoparticle; Polysaccharide;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The toxic effect of Aβ42 induced by copper nanoparticle (Cu NPs) was studied by atomic force microscopy (AFM), circular dichroism (CD) spectroscopy, and Thioflavin T (ThT) fluorescence technique. Five hundred nanometers of copper nanoparticle capped with polyvinylpyrrolidone (PVP) was used to evaluate the aggregation and fibrils of Aβ42. The morphologies of Aβ42 incubated in the presence of Cu NPs changed gradually. The aggregation and fibrils were observed in AFM images. However, in the presence of polysaccharides, the Cu NPs-induced fibrillation of Aβ42 was inhibited. Interestingly, the formed Cu NPs–polysaccharides complexes can even remodel the preformed Aβ42 fibrils into the low neurotoxic amorphous aggregates, which were maybe ascribed to the higher affinity of polysaccharides for Aβ42 than Cu NPs. Besides, it was found that the binding constant of Cu NPs to Aβ42 is smaller than that of polysaccharides. The relationship among polysaccharides, copper nanoparticle, and Aβ42 morphologies and its neurotoxicity were discussed, and the binding force was analyzed.
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页码：1557 / 1566
页数：9
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