Transcriptome sequencing analysis of porcine alveolar macrophages infected with PRRSV strains to elucidate virus pathogenicity and immune evasion strategies

被引：2

作者：

Wang F.-X. ^{[1
]}

Liu X. ^{[2
]}

Wu H. ^{[3
]}

Wen Y.-J. ^{[1
,2
]}

机构：

[1] College of Veterinary Medicine, Key Laboratory for Clinical Diagnosis and Treatment of Animal Diseases of Ministry of Agriculture, Inner Mongolia Agricultural University, No. 29, E’erduosi east street, Saihan Region, Hohhot

[2] State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Jingyue Economic and Technological Development Zone, No. 4899, Juye Avenue, Jilin, Ch

[3] Sinovet (Beijing) Biotechnology Co., Ltd, B302 Zhongguancun Biomedical Park, 5 Shangdikaituo Road, Beijing

来源：

VirusDisease | 2021年 / 32卷 / 3期

基金：

中国国家自然科学基金;

关键词：

Differentially expressed genes; PAM; PRRSV; RNA-seq; Transcriptome;

D O I：

10.1007/s13337-021-00724-0

中图分类号：

学科分类号：

摘要：

Highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) causes a serious disease to the swine industry worldwide. To understand the mechanisms of HP-PRRSV infection, RNA-seq-based transcriptome analyses were performed on porcine alveolar macrophages (PAMs) infected with a HP-PRRSV strain (TJ), a less virulent strain of a classical lineage (CH-1a), and a vaccine strain TJM-F92. Gene ontology, Kyoto Encyclopedia of Genes and Genomes analyses indicate that TJM-F92 led to significant up-regulation of gene expression for proteins associated with membrane-bound organelles. The differentially expressed genes of HP-PRRSV TJ-infected PAM cells were up-regulated in the special G-protein coupled receptor. The six cytokines were tested by real time Reverse Transcription-Polymerase Chain Reaction (RT-PCR). The relative expression levels showed the same trend of expression difference. Significant up-regulation of TMEM173 plays an important role in the cytosolic DNA-sensing pathway and the RIG-I-like receptor signaling pathway in TJM-F92 infected PAM cells. These data provide new insight into PRRSV pathogenicity and immune evasion strategies. © 2021, Indian Virological Society.

引用

页码：559 / 567

页数：8

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