Investigation of peak compression effects in packed column supercritical fluid chromatography using chemometrics

In this paper chemometrics have been used to study and characterize peak compression phenomena in packed column SFC. A carbon dioxide/2-methyl-1-propanol mobile phase was used in the experimental design (modifier concentration, temperature and pressure) and modelling part of the investigation. A cubic interaction term was needed in the model to obtain a reasonable fit, suggesting that all three parameters are of significance in terms of controlling peak compression. At the optimum conditions derived from the model a narrow peak was obtained as predicted.