Expression of human immunodeficiency virus genes using baculovirus expression system

被引：2

作者：

Yong Kang C. ^{[1
]}

机构：

[1] Faculty of Science, Western Science Centre, University of Western Ontario, London, N6A 5B7, ON

来源：

Molecular Biotechnology | 1997年 / 8卷 / 2期

基金：

英国医学研究理事会;

关键词：

AIDs; baculovirus expression system; gene expression; Human immunodeficiency virus; vaccines; viral proteins;

D O I：

10.1007/BF02752262

中图分类号：

学科分类号：

摘要：

The structural protein genes of HIV-1 and HIV-2 have been expressed inSpodoptera frugiperda (SF) cells using baculovirus expression system. The noncoding flanking sequences of HIV structural genes were removed and a putative ribosome binding site was placed in front of the open reading frame of each gene by using crossover linker mutagenesis. The coding sequences of thegag, pol, env, andvif proteins were inserted intoAutographa californica nuclear polyhedrosis virus (AcNPV) so that HIV genes were under the control of the AcNPV polyhedrin promoter. All recombinant AcNPV-infected SF cells express high levels of HIV structural proteins. Detailed strategies of recombinant AcNPV construction for high level protein expression are presented. © 1997, Humana Press Inc.

引用

页码：173 / 187

页数：14

共 24 条

[11]

Mondelli M., Vergami G.M., Alberti A., Vergami D., Et al., Specificity of T lymphocyte cytotoxicity to autologous hepatocytes in chronic hepatitis B virus infection: evidence that T cells are directed against HBV core antigen expressed on hepatocytes, J. Immunol., 129, pp. 2773-2778, (1982)

[12]

Walker B.D., Flexner C., Paradis T.J., Hirsch M.S., HIV-1 reverse transcriptase is a target for cytotoxic T lymphocytes in infected individuals, Science, 240, pp. 64-66, (1988)

[13]

Walker B.D., Chakrabarti S., Moss B., Paradis T.J., Et al., HIV-specific cytotoxic T lymphocytes in seropositive individuals, Nature (Lond.), 328, pp. 345-348, (1987)

[14]

Imberti L., Sottini A., Bettinardi A., Puoti M., Primi D., Selective depletion in HIV infection of T cells that bear specific T cell receptor V<sub>β</sub> sequences, Science, 254, pp. 860-862, (1991)

[15]

Matsuura Y., Possee R.D., Overton H.A., Bishop D.H.L., Baculovirus expression vectors: the requirements for high level expression of proteins, including glycoproteins, J. Gen. Virol., 68, pp. 1233-1250, (1987)

[16]

Ratner L., Haseltine W., Patarca R., Livak K.J., Et al., Complete nucleotide sequence of the AIDS virus, HTLV-III, Nature (Lond.), 313, pp. 277-284, (1985)

[17]

Sung W.L., Zahab D.M., MacDonald C.A., Tam C.S., Synthesis of mutant parathyroid hormone genes via site-specific recombination directed by crossover linkers, Gene, 47, pp. 261-267, (1986)

[18]

Garson K., Percival H., Kang C.-Y., The N-terminal env-derived amino acids of v-rel are required for full transforming activity, Virology, 177, pp. 106-115, (1990)

[19]

Franchini G., Collati E., Arya A., Fenyo E., Biberfeld G., Zagury J.F., Kanki P.J., Wong-Staal F., Gallo R.C., Genetic analysis of a new subgroup of human and simian T-lymphotropic retroviruses HTLV-IV LAV-2 SBL-6669 and STLV-III-AGM, AIDS Res. Human Retroviruses, 3, pp. 11-18, (1987)

[20]

Luo L., Li Y., Kang C.-Y., Expression ofgag precursor protein and secretion of virus-likegag particles of HIV-2 from recombinant baculo-virus-infected insect cells, Virology, 179, pp. 874-880, (1990)

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