Changes in 18S-25S rDNA in a tissue culture of some Gentiana L. species

被引：0
作者：
V. M. Mel’nyk
I. O. Andreev
K. V. Spiridonova
N. M. Strashnyuk
V. A. Kunakh
机构：
[1] National Academy of Sciences of Ukraine,Institute of Molecular Biology and Genetics
[2] Volodymyr Gnatyuk National Pedagogical University of Ternopil,undefined
来源：
Cytology and Genetics | 2007年 / 41卷
关键词：
Blot Hybridization; Callus Tissue; Intact Plant; 50th Passage; 23rd Passage;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
18S-25S rDNA of intact plants and tissue cultures of G. acaulis, G. punctata, and G. lutea are studied by blot hybridization. A decrease in the number of repeats of ribosomal DNA by comparison with the plants is established in the callus tissues. Unlike other species, G. lutea exhibits intragenome heterogeneity of rRNA genes as well as qualitative changes in rDNA in tissue cultures, in particular, the appearance of altered repeats. It is suggested that there exists an association between these features of the structure of rRNA genes and their rearrangement in vitro.
引用
收藏
页码：82 / 85
页数：3
相关论文
共 24 条
  • [1] Kupriyanova N.S.(2000)Conservatism and Variability of Ribosome Eukaryotic DNA Molekulyarn. biologiya 34 753-765
  • [2] Volkov R.A.(2003)Plant rDNA: Organization, Evolution, Application Tsitol. Genet. 37 72-78
  • [3] Panchuk I.I.(1999)Variability of Plant Genome in Process of Dedifferentiation and Callus Formation Fiziologiya rasteniy 46 919-929
  • [4] Borysyuk L.G.(2000)Genetic Fidelity of Organized Meristem-Derived Micropropagated Plants: A Critical Reappraisal In Vitro Cell. Dev. Biol. (Plant) 36 319-330
  • [5] Borysyuk M.V.(2004)Introduction to Fiziologiya i biokhimiya kul’tur. rastenii 36 327-334
  • [6] Kunakh V.A.(1985) Culture of Certain Species of the Genus Plant Mol. Biol. 5 69-76
  • [7] Rani V.(1987) L. Plant Mol. Biol. 8 3-12
  • [8] Raina S.N.(1987)Extraction of DNA from Milligram Amounts of Fresh Herbarium and Mummified Plant Tissues Plant Mol. Biol. 9 509-520
  • [9] Strashnyuk N.M.(2003)Structure and Variation in Ribosomal RNA Genes of Peas Tsitol. Genet. 37 65-71
  • [10] Gritsak L.R.(undefined)Ribosomal RNA Genes in Plants: Variability in Copy Number and in the Intergenic Spacer undefined undefined undefined-undefined
123