Human Umbilical Cord Mesenchymal Stromal Cells Support Viability of Umbilical Cord Blood Hematopoietic Stem Cells but not the “Stemness” of Their Progeny in Co-Culture

被引：0
作者：
Yu. A. Romanov
N. E. Volgina
E. E. Balashova
N. V. Kabaeva
T. N. Dugina
G. T. Sukhikh
机构：
[1] Ministry of Health of the Russian Federation,Russian Cardiology Research
[2] V. I. Kulakov Research Center of Obstetrics,and
[3] Gynecology,Production Complex
[4] and Perinatology,undefined
[5] Ministry of Health of the Russian Federation,undefined
[6] CryoCenter Cord Blood Bank,undefined
来源：
Bulletin of Experimental Biology and Medicine | 2017年 / 163卷
关键词：
mesenchymal stromal cells; umbilical cord; hematopoietic stem cells; umbilical cord blood; co-culturing;
D O I：
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学科分类号：
摘要：
Cell—cell interactions and the ability of mesenchymal stromal cells to support the expansion of hematopoietic progenitor cells were studied in co-culture of human umbilical cord tissue-derived mesenchymal stromal cells and nucleated umbilical cord blood cells. It was found that hematopoietic stem cells from the umbilical cord blood are capable to adhere to mesenchymal stromal cells and proliferate during 3-4 weeks in co-culture. However, despite the formation of hematopoietic foci and accumulation of CD34+ and CD133+ cells in the adherent cell fraction, the ability of newly generated blood cells to form colonies in semi-solid culture medium was appreciably reduced. These findings suggest that human umbilical cord tissue-derived mesenchymal stromal cells display a weak capability to support the “stemness” of hematopoietic stem cell progeny despite long-term maintenance of their viability and proliferation.
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页码：523 / 527
页数：4
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