Transcription factor YY1 is essential for iNKT cell development

被引：0
作者：
Xijun Ou
Jianxin Huo
Yuhan Huang
Yan-Feng Li
Shengli Xu
Kong-Peng Lam
机构：
[1] Agency for Science,Immunology Group, Bioprocessing Technology Institute
[2] Technology and Research,Department of Biology
[3] Southern University of Science and Technology,Department of Physiology
[4] National University of Singapore,Department of Microbiology and Immunology
[5] National University of Singapore,Department of Pediatrics, Yong Loo Lin School of Medicine
[6] National University of Singapore,School of Biological Sciences
[7] Nanyang Technological University,undefined
来源：
Cellular & Molecular Immunology | 2019年 / 16卷
关键词：
NKT cell; PLZF; YY1; Zbtb16;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Invariant natural killer T (iNKT) cells develop from CD4+CD8+ double-positive (DP) thymocytes and express an invariant Vα14–Jα18 T-cell receptor (TCR) α-chain. Generation of these cells requires the prolonged survival of DP thymocytes to allow for Vα14–Jα18 gene rearrangements and strong TCR signaling to induce the expression of the iNKT lineage-specific transcription factor PLZF. Here, we report that the transcription factor Yin Yang 1 (YY1) is essential for iNKT cell formation. Thymocytes lacking YY1 displayed a block in iNKT cell development at the earliest progenitor stage. YY1-deficient thymocytes underwent normal Vα14–Jα18 gene rearrangements, but exhibited impaired cell survival. Deletion of the apoptotic protein BIM failed to rescue the defect in iNKT cell generation. Chromatin immunoprecipitation and deep-sequencing experiments demonstrated that YY1 directly binds and activates the promoter of the Plzf gene. Thus, YY1 plays essential roles in iNKT cell development by coordinately regulating cell survival and PLZF expression.
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页码：547 / 556
页数：9
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