Isolation and characterization of microsatellite markers for Ligularia hodgsonii Hook. (Asteraceae)

被引:0
作者
Changli Mao
Yuezhi Pan
Jinfeng Wang
Xun Gong
机构
[1] Chinese Academy of Sciences,Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany
来源
Conservation Genetics | 2009年 / 10卷
关键词
FIASCO; Microsatellite markers; Polymorphism;
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中图分类号
学科分类号
摘要
Ligularia hodgsonii is one of widely distributed Ligularia species in south China, central China, Far East area of Russia and Japan. In this study, we described the development of 14 microsatellite markers from the genome of L. hodgsonii using the FIASCO protocol. Polymorphism of each locus was assessed in 30 adult individuals of the Ligularia. The average allele number of the microsatellites was 3.0 per locus, ranging from 2 to 5. The observed (HO) and expected (HE) heterozygosities varied from 0.2000 to 0.7333 and from 0.3910 to 0.7598. The marker transferability of the 14 primer pairs was tested on other two congeneric species that also occur in China.
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页码:1853 / 1855
页数:2
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