Development of sequence characterized amplified region (SCAR) primers for the detection of Phyto.5.2, a major QTL for resistance to Phytophthora capsici Leon. in pepper

被引:0
作者
E. A. Quirin
E. A. Ogundiwin
J. P. Prince
M. Mazourek
M. O. Briggs
T. S. Chlanda
K-T Kim
M. Falise
B-C Kang
M. M. Jahn
机构
[1] Cornell University,Department of Plant Breeding and Genetics
[2] California State University,Department of Biology
来源
Theoretical and Applied Genetics | 2005年 / 110卷
关键词
Quantitative Trait Locus; Phytophthora; Major Quantitative Trait Locus; Amplify Fragment Length Polymorphic; Sequence Characterize Amplify Region Marker;
D O I
暂无
中图分类号
学科分类号
摘要
Phytophthora capsici causes devastating disease on many crop species, including Capsicum. Resistance in Capsicum annuum is genetically and physiologically complex. A panel of Capsicum germplasm that included genotypes from both C. annuum and C. chinense showing highly resistant, highly susceptible and intermediate or tolerant responses to the pathogen, respectively, was screened with a series of randomly amplified polymorphic sequence primers to determine which genomic regions contribute to the highest level of resistance. One primer, OpD04, amplified a single band only in those C. annuum and C. chinense genotypes showing the highest level of resistance. The amplified product was cloned, sequenced and used to design longer primers in order to generate a sequence characterized amplified region marker which was then mapped in a reference mapping population and a screened population segregating for resistance to P. capsici. These primers were observed to define a locus on pepper chromosome 5 tightly linked to Phyto.5.2, one of six quantitative trait loci (QTL) previously reported to contribute to P. capsici resistance. These results indicate that the Phyto.5.2 QTL may be widely distributed in highly resistant germplasm and provide improved resolution for this QTL. This work also defines the first breeding tools for this system, allowing for the rapid selection of genotypes likely to be highly resistant to P. capsici.
引用
收藏
页码:605 / 612
页数:7
相关论文
共 85 条
[21]  
Green M(1999)An integrated genetic linage map of pepper ( Plant Dis 83 1080-undefined
[22]  
Abrahamson S(2001) spp.) Molecular cloning a-undefined
[23]  
Barlow L(2003)Defense response genes co-localize with quantitative disease resistance loci in pepper Theor Appl Genet 106 1473-undefined
[24]  
Daley V(2004)Etude de deux nouvelles sources de resistances a Theor Appl Genet 109 342-undefined
[25]  
Lincoln A(1999) Leon. chez le piment: confirmation de l’existence de trois composantes distinctes dans la resistance J Am Soc Hortic Sci 124 14-undefined
[26]  
Newburg KS(undefined)A versatile and high-yielding protocol for the preparation of genomic DNA from undefined undefined undefined-undefined
[27]  
Lefebvre VK(undefined) spp. (pepper) undefined undefined undefined-undefined
[28]  
Palloix JR(undefined)Ecologically based approaches to management of undefined undefined undefined-undefined
[29]  
Lefebvre R(undefined) blight on bell pepper undefined undefined undefined-undefined
[30]  
Livingstone MM(undefined)Comparative mapping of undefined undefined undefined-undefined