Comparison of two eukaryotic systems for the expression of VP6 protein of rotavirus specie A: transient gene expression in HEK293-T cells and insect cell-baculovirus system

被引：0

作者：

Haroldo Cid da Silva Junior

Sérgio da Silva e Mouta Junior

Marcos César Lima de Mendonça

Mirian Claudia de Souza Pereira

Alanderson da Rocha Nogueira

Maria Luiza Borges de Azevedo

José Paulo Gagliardi Leite

Márcia Terezinha Baroni de Moraes

机构：

[1] Fundação Oswaldo Cruz. Av. Brasil,Laboratório de Virologia Comparada e Ambiental, Instituto Oswaldo Cruz

[2] Fundação Oswaldo Cruz,Laboratório de Virologia Molecular, Instituto Oswaldo Cruz

[3] Fundação Oswaldo Cruz,Laboratório de Ultra

[4] Bio-Manguinhos/Fundação Oswaldo Cruz,estrutura Celular, Instituto Oswaldo Cruz

来源：

Biotechnology Letters | 2012年 / 34卷

关键词：

Rotavirus A; VP6 protein; HEK293-T cells; Sf9 cells; Diagnosis; Vaccine;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

The VP6 protein of rotavirus A (RVA) is a target antigen used for diagnostic assays and also for the development of new RVA vaccines. We have compared the expression of VP6 protein in human embryonic kidney (HEK293-T) cells with results obtained using a well-established insect cell-baculovirus system. The recombinant VP6 (rVP6) expressed in HEK293-T cells did not present degradation and also retained the ability to form trimers. In the insect cell-baculovirus system, rVP6 was expressed at higher levels and with protein degradation as well as partial loss of ability to form trimers was observed. Therefore, HEK293-T cells represent a less laborious alternative system than insect cells for expression of rVP6 from human RVA.

引用

页码：1623 / 1627

页数：4