The LKEKK synthetic peptide as a ligand of rat intestinal epithelial cell membranes

被引：0
|
作者：
E. V. Navolotskaya
V. B. Sadovnikov
D. V. Zinchenko
V. I. Vladimirov
Y. A. Zolotarev
A. A. Kolobov
机构：
[1] Branch of Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences,
[2] Institute of Molecular Genetics of the Russian Academy of Science,undefined
[3] State Research Center for Institute of Highly Pure Biopreparations of Federal Biomedical Agency,undefined
来源：
Russian Journal of Bioorganic Chemistry | 2016年 / 42卷
关键词：
peptides; receptors; thymosin-α; interferon-α; cholera toxin B subunit;
D O I：
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学科分类号：
摘要：
A tritium-labeled synthetic LKEKK pentapeptide corresponding to the sequences 16–20 of human thymosin-α1 and 131–135 of human interferon-α2 was obtained with a specific activity of 28 Ci/mmol. [3H]LKEKK was found to bind with high affinity (Kd 3.7 ± 0.3 nM) to the membranes isolated from epithelial cells of rat small intestinal mucosa. The trypsin treatment of the membranes did not affect the binding, thus supporting the nonprotein nature of the peptide receptor. The binding of the labeled peptide was inhibited by unlabeled thymosin-α1, interferon-α2, and cholera toxin B subunit (Ki 4.2 ± 0.4, 3.5 ± 0.3, and 4.7 ± 0.3 nM respectively). The pentapeptide did not affect the adenylate cyclase activity within the concentration range of 1–1000 nM.
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页码：479 / 483
页数：4
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