Development and Validation of a Normal-Phase HPTLC-Densitometric Method for the Quantitative Analysis of Fluconazole in Tablets

被引：0
|
作者：
Danstan Hipolite Shewiyo
Eliangiringa Kaale
Peter Gasper Risha
Hiiti Baran Sillo
Bieke Dejaegher
J. Smeyers-Verbeke
Yvan Vander Heyden
机构：
[1] Tanzania Food and Drugs Authority,Analytical Chemistry and Pharmaceutical Technology, Center for Pharmaceutical Research
[2] Muhimbili University of Health and Allied Sciences,undefined
[3] Tanzania Food and Drugs Authority,undefined
[4] Vrije Universiteit Brussel,undefined
来源：
JPC – Journal of Planar Chromatography – Modern TLC | 2011年 / 24卷
关键词：
Normal-phase high-performance thin-layer chromatography (NP HPTLC); Fluconazole; Densitometry; Validation;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
This paper presents the development and validation of an improved method for the analysis of fluconazole using high-performance thinlayer chromatography (HPTLC) with densitometric detection. Separation was performed on silica gel 60 F254 plates. The mobile phase is comprised of ethyl acetate-methanol-ammonia-diaminoethane (85:10:5:0.5 v/v/v/v). Detection wavelength was 216 nm and the RF value obtained was 0.4 ± 0.03. An F-test indicated that the calibration graph was linear at the evaluated concentration range. The %RSD for repeatability and intermediate precision were 1.60 and 3.05, respectively. The mean percentage recovery for the trueness was 99.1 ± 2.1%. This method was successfully used to analyze the fluconazole content in marketed tablet samples.
引用
收藏
页码：529 / 533
页数：4
相关论文
共 50 条
  • [41] Simultaneous Estimation of Lesinurad and Allopurinol in the New FDA-Approved Products Using a Greener Normal-Phase HPTLC Method: Greenness Assessment Using the NEMI, Analytical Eco-Score, ChlorTox, and AGREE Methods
    Alam, Prawez
    Shakeel, Faiyaz
    Alshehri, Sultan
    Iqbal, Muzaffar
    Foudah, Ahmed I.
    Aljarba, Tariq M.
    Abdel Bar, Fatma
    Alqarni, Mohammed H.
    ACS SUSTAINABLE CHEMISTRY & ENGINEERING, 2024, 12 (04) : 1526 - 1535
  • [42] Development and Validation of New RP-UPLC Method for the Quantitative Analysis of Mycophenolate in Tablet Dosage Form
    Abhilash, P. Raja
    Kumar, K. Vasanth
    Kumar, V. Sateesh
    BRITISH JOURNAL OF PHARMACEUTICAL RESEARCH, 2013, 3 (03): : 363 - 373
  • [43] Quantitative Analysis of Norfloxacin in β-Cyclodextrin Inclusion Complexes—Development and Validation of a Stability-indicating HPLC Method
    Cassiana Mendes
    Aline Buttchevitz
    Jéssica Henriques Kruger
    Larissa Sakis Bernardi
    Paulo Renato Oliveira
    Marcos Antônio Segatto Silva
    Analytical Sciences, 2015, 31 : 1083 - 1089
  • [44] DEVELOPMENT AND VALIDATION OF A STABILITY-INDICATING HPLC METHOD FOR ANALYSIS OF ZIDOVUDINE (ZDV) IN BULK DRUG AND IN VITRO RELEASE STUDIES OF TABLETS
    Mandloi, Dileep
    Tripathi, Priyanka
    Mohanraj, Palanikar
    Chauhan, Nagendra Singh
    Patel, Jay Ram
    JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2011, 34 (08) : 601 - 612
  • [45] DEVELOPMENT AND VALIDATION OF A STABILITY-INDICATING REVERSED PHASE ULTRA PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR THE QUANTITATIVE ANALYSIS OF RALOXIFENE HYDROCHLORIDE IN PHARMACEUTICAL DOSAGE FORM
    Saini, Deepa
    Baboota, Sanjula
    Ali, Mushir
    Patel, Harilal
    Jain, Pankaj
    Neerumulla, Sudhakar
    Ali, Javed
    JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2012, 35 (1-4) : 162 - 173
  • [46] Development and validation of a reversed-phase liquid chromatographic method for analysis of rofecoxib in human plasma
    Krishnaiah, YSR
    Rao, GS
    Bhaskar, P
    Shyale, SS
    ASIAN JOURNAL OF CHEMISTRY, 2003, 15 (02) : 945 - 948
  • [47] Development and Validation of a Fast Reversed Phase Liquid Chromatographic Method for the Analysis of Ethionamide in Dosage Forms
    Kahsay, Getu
    Shraim, Fairouz
    Lin, Qi
    Van Schepdael, Ann
    Adams, Erwin
    CURRENT PHARMACEUTICAL ANALYSIS, 2018, 14 (03) : 312 - 319
  • [48] DEVELOPMENT AND VALIDATION OF FAST REVERSED-PHASE HPLC METHOD FOR ANALYSIS OF ESOMEPRAZOLE IN RABBIT PLASMA
    Ranjha, Nazar Muhammad
    Rashid, Zermina
    Razzaq, Rabia
    Raza, Hina
    Hanif, Muhammad
    Mahmood, Asif
    ACTA POLONIAE PHARMACEUTICA, 2017, 74 (02): : 385 - 391
  • [49] Development of a Densitometric High-Performance Thin-Layer Chromatographic Method for the Quantitative Analysis of Biomarker Lupeol in the Leaves of Different Species of Genus Ficus
    Perwez Alam
    Omer A. Basudan
    Nasir A. Siddiqui
    Adnan J. Al-Rehaily
    Saleh I. Alqasoumi
    Maged S. Abdel-Kader
    Abd El Raheim M. Donia
    Prawez Alam
    JPC – Journal of Planar Chromatography – Modern TLC, 2015, 28 : 30 - 35
  • [50] Development of a Densitometric High-Performance Thin-Layer Chromatographic Method for the Quantitative Analysis of Biomarker Lupeol in the Leaves of Different Species of Genus Ficus
    Alam, Perwez
    Basudan, Omer A.
    Siddiqui, Nasir A.
    Al-Rehaily, Adnan J.
    Alqasoumi, Saleh I.
    Abdel-Kader, Maged S.
    Donia, Abd El Raheim M.
    Alam, Prawez
    JPC-JOURNAL OF PLANAR CHROMATOGRAPHY-MODERN TLC, 2015, 28 (01) : 30 - 35
12345