Development and Validation of a Normal-Phase HPTLC-Densitometric Method for the Quantitative Analysis of Fluconazole in Tablets

被引：0
|
作者：
Danstan Hipolite Shewiyo
Eliangiringa Kaale
Peter Gasper Risha
Hiiti Baran Sillo
Bieke Dejaegher
J. Smeyers-Verbeke
Yvan Vander Heyden
机构：
[1] Tanzania Food and Drugs Authority,Analytical Chemistry and Pharmaceutical Technology, Center for Pharmaceutical Research
[2] Muhimbili University of Health and Allied Sciences,undefined
[3] Tanzania Food and Drugs Authority,undefined
[4] Vrije Universiteit Brussel,undefined
来源：
JPC – Journal of Planar Chromatography – Modern TLC | 2011年 / 24卷
关键词：
Normal-phase high-performance thin-layer chromatography (NP HPTLC); Fluconazole; Densitometry; Validation;
D O I：
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中图分类号：
学科分类号：
摘要：
This paper presents the development and validation of an improved method for the analysis of fluconazole using high-performance thinlayer chromatography (HPTLC) with densitometric detection. Separation was performed on silica gel 60 F254 plates. The mobile phase is comprised of ethyl acetate-methanol-ammonia-diaminoethane (85:10:5:0.5 v/v/v/v). Detection wavelength was 216 nm and the RF value obtained was 0.4 ± 0.03. An F-test indicated that the calibration graph was linear at the evaluated concentration range. The %RSD for repeatability and intermediate precision were 1.60 and 3.05, respectively. The mean percentage recovery for the trueness was 99.1 ± 2.1%. This method was successfully used to analyze the fluconazole content in marketed tablet samples.
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页码：529 / 533
页数：4
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