Murine polyomavirus-like particles induce maturation of bone marrow-derived dendritic cells and proliferation of T cells

被引：0
作者：
Thomas Bickert
Gisela Wohlleben
Marc Brinkman
Claudia M. Trujillo-Vargas
Claus Ruehland
Christian O. A. Reiser
Juergen Hess
Klaus J. Erb
机构：
[1] University of Wuerzburg,Center for Infectious Diseases and Institute for Molecular Infection Biology
[2] responsif GmbH,Department of Immunology
[3] Bernhard Nocht Institute for Tropical Medicine,Institute for Virology and Immunobiology
[4] University of Wuerzburg,Grupo de Immunodeficiencias Primarias
[5] Universidad de Antioquia,undefined
来源：
Medical Microbiology and Immunology | 2007年 / 196卷
关键词：
Virus-like particles; Dendritic cells; Maturation; T cell proliferation; Chimeric PLPs;
D O I：
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学科分类号：
摘要：
We analysed the effects of murine polyomavirus-like particles (PLPs) on bone marrow-derived dendritic cells (BMDCs) and T cells in vitro. BMDCs activated with PLPs up-regulated CD40, CD80, CD86 and major histocompatibility complex (MHC) class II surface markers and produced proinflammatory cytokines. Chimeric PLPs [expressing the ovalbumin (OVA)-peptides OVA257–264 or OVA323–339], but not wildtype PLPs, activated OVA-specific CD8 T cells and OVA-specific CD4 T cells, respectively, indicating both MHC class I and II presentation of the peptides by antigen-presenting cells. Our results suggest that PLPs may be used as vaccine adjuvants priming dendritic cells to induce potent T cell responses.
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页码：31 / 39
页数：8
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共 177 条
  • [1] Abbing A(2004)Efficient intracellular delivery of a protein and a low molecular weight substance via recombinant polyomavirus-like particles J Biol Chem 279 27410-27421
  • [2] Blaschke UK(2001)Bacterial carriers and virus-like-particles as antigen delivery devices: role of dendritic cells in antigen presentation Curr Drug Targets Infect Disord 1 287-302
  • [3] Grein S(2002)Virus-like particles: a new family of delivery systems Expert Rev Vaccines 1 101-109
  • [4] Kretschmar M(2005)Polyomavirus EGFP-pseudocapsids: analysis of model particles for introduction of proteins and peptides into mammalian cells FEBS Lett 579 6549-6558
  • [5] Stark CMB(2004)Recombinant murine polyoma virus-like-particles induce protective anti-tumour immunity Lett Drug Des Discov 1 137-147
  • [6] Thies MJW(2005)Beneficial therapeutic effects with different particulate structures of murine polyomavirus VP1-coat protein carrying self or non-self CD8 T cell epitopes against murine melanoma Cancer Immunol Immunother 54 611-622
  • [7] Walter J(2004)Transfer of antigen between migrating and lymph node-resident DCs in peripheral T cell tolerance and immunity Trends Immunol 25 655-658
  • [8] Weigand M(1999)Vaccinia virus inhibits the maturation of human dendritic cells: a novel mechanism of immune evasion J Immunol 163 6762-6768
  • [9] Woith DC(2003)Recombinant human heat shock protein 60 does not induce the release of tumor necrosis factor alpha from murine macrophages J Biol Chem 278 22523-22529
  • [10] Hess J(2003)Cell penetration and trafficking of polyomavirus J Virol 77 2615-2622
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