Serial blood cultures from canine stored whole blood held at room temperature for 24 h

被引:2
作者
Beymer J.S. [1 ]
Rudloff E. [1 ]
Kirby R. [1 ]
Novicki T.J. [2 ]
Moore F.M. [2 ]
机构
[1] Animal Emergency Center and Specialty Services, Glendale, WI 53209
[2] Marshfield Clinic Laboratories, Marshfield, WI 54449
关键词
Bacterial contamination; Blood cultures; Blood transfusion; Canine;
D O I
10.1007/s00580-008-0801-8
中图分类号
学科分类号
摘要
To determine if 30- to 35-day-old units of canine stored whole blood held at room temperature over 24 h will reveal bacterial contamination when exposed to repeated sampling. The design of the study is prospective, controlled. Ten units of stored whole blood from ten client owned volunteer blood donor dogs were used. A sterile blood bag spike was inserted aseptically as a port for repeated sampling for culture into ten separate units of 30- to 35-day-old canine stored whole blood held at room temperature for 24 h. Aerobic and anaerobic bacterial culture samples were collected every 4 h over 24 h. No bacterial growth was detected in any sample at any time point from the ten bags used. Canine stored whole blood, collected and stored for 30 to 35 days according to accepted transfusion guidelines, can be aseptically spiked and held at room temperature for 24 h without evidence of bacterial contamination or proliferation. © Springer-Verlag London Limited 2009.
引用
收藏
页码:279 / 281
页数:2
相关论文
共 14 条
[1]  
Bracker K., Drellich S., Transfusion reactions, Compend Contin Educ Vet, 7, pp. 500-511, (2005)
[2]  
Brecher M., Hay S., Bacterial contamination of blood components, Clin Microbiol Rev, 1, pp. 195-204, (2005)
[3]  
Brecher M., Hay S., Rothenberg S., Validation of BacTAlert plastic culture bottles for use in testing of whole blood derived leukoreduced platelet rich plasma products, Transfusion, 44, pp. 1174-1178, (2004)
[4]  
Chiaramonte D., Blood-component therapy: Selection, administration and monitoring, Clin Tech Small Anim, 19, pp. 63-67, (2004)
[5]  
Dodd R., Bacterial contamination and transfusion safety: Experience in the United States, Transfus Clin Biol, 1, pp. 6-9, (2003)
[6]  
Fang C., Chambers L., Kennedy J., Et al., Detection of bacterial contamination in apheresis platelet products: American Red Cross experience, Transfusion, 12, pp. 1845-1852, (2005)
[7]  
Hale A., Practical Canine blood transfusion, NAVC Clinician's Brief, 9, pp. 37-40, (2005)
[8]  
Haldane S., Roberts J., Marks S., Transfusion medicine, Compend Contin Educ, 7, pp. 502-517, (2004)
[9]  
Hillyer C., Josephson C., Blajchman M., Et al., Bacterial contamination of blood components: Risks, Strategies, and Regulation, Hematology (Am Soc Hematol Educ Program), pp. 575-589, (2003)
[10]  
Kopko P., Hollan P., Mechanisms of severe transfusion reactions, Transfus Clin Biol, 3, pp. 278-281, (2001)