Generation of genetic engineering monoclonal antibodies against prion protein

被引：0
作者：
Yin-Xia Huang
Jun Han
Chen-Fang Dong
Li Sun
Chen Gao
Xiao-Fan Wang
Lu Han
Wei Zhou
Bao-Yun Zhang
Hui-Ying Jiang
Mi-Fang Liang
Xiao-Ping Dong
机构：
[1] Chinese Center for Disease Control and Prevention,State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention
来源：
Medical Microbiology and Immunology | 2007年 / 196卷
关键词：
TSEs; Phage display; Monoclonal antibodies; Baculovirus expression system;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Two strains of Fab monoclonal antibodies (mAbs) against prion protein, designated as IV-66 and IV-78, were selected from the phage display libraries. The gene sequences encoding the light κ chain and heavy Fd chain of IV-78 were inserted into a baculovirus expression cassette vector for mouse IgG expression. Western blot, Dot-ELISA and immunoprecipitation confirmed that these Fab and IgG mAbs reacted well with the recombinant hamster and human PrP proteins expressed in prokaryotic and in mammalian cells and PrPSc from scrapie-infected hamsters. It demonstrates that mAbs against prion protein are successfully generated by phage-display technique.
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页码：241 / 246
页数：5
相关论文
共 180 条
[11]  
Prusiner SB(2006)Antibody-mediated neuronal apoptosis: therapeutic implications for prion diseases Immunol Lett 105 123-126
[12]  
Soto C(1991)Making antibody fragments using phage display libraries Nature 352 624-628
[13]  
Zou WQ(1994)Human antibodies from combinatorial libraries Adv Immunol 57 191-280
[14]  
Zheng J(1990)High level production of a functional immunoglobulin heterodimer in a baculovirus expression system Proc Natl Acad Sci USA 87 3942-3946
[15]  
Gray DM(1992)Production of a functional monoclonal antibody recognizing human colorectal carcinoma cells from a baculovirus expression system J Immunol Methods 151 201-208
[16]  
Gambetti P(1995)Design of cassette baculovirus vectors for the production of therapeutic antibodies in sect cells Immunotechnology 1 189-196
[17]  
Chen SG(1997)Baculovirus expression of a human G2-specific, neutralizing IgG monoclonal antibody to Puumala virus Virology 235 252-260
[18]  
Miyamoto K(2002)Expression of PrP(C) as HIS-fusion form in a baculovirus system and conversion of expressed PrP-sen to PrP-res in a cell-free system Virus Res 87 145-153
[19]  
Nakamura N(1993)Human monoclonal antibodies against a plethora of viral pathogens from single combinatorial libraries Proc Natl Acad Sci USA 90 4141-4145
[20]  
Aosasa M(1991)Assembly of combinatorial antibody libraries on phage surfaces:the gene III site Proc Natl Acad Sci USA 88 7978-7982
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