Generation of genetic engineering monoclonal antibodies against prion protein

被引：0
作者：
Yin-Xia Huang
Jun Han
Chen-Fang Dong
Li Sun
Chen Gao
Xiao-Fan Wang
Lu Han
Wei Zhou
Bao-Yun Zhang
Hui-Ying Jiang
Mi-Fang Liang
Xiao-Ping Dong
机构：
[1] Chinese Center for Disease Control and Prevention,State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention
来源：
Medical Microbiology and Immunology | 2007年 / 196卷
关键词：
TSEs; Phage display; Monoclonal antibodies; Baculovirus expression system;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Two strains of Fab monoclonal antibodies (mAbs) against prion protein, designated as IV-66 and IV-78, were selected from the phage display libraries. The gene sequences encoding the light κ chain and heavy Fd chain of IV-78 were inserted into a baculovirus expression cassette vector for mouse IgG expression. Western blot, Dot-ELISA and immunoprecipitation confirmed that these Fab and IgG mAbs reacted well with the recombinant hamster and human PrP proteins expressed in prokaryotic and in mammalian cells and PrPSc from scrapie-infected hamsters. It demonstrates that mAbs against prion protein are successfully generated by phage-display technique.
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页码：241 / 246
页数：5
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