Identification and characterization of a novel phage display-derived peptide with affinity for human brain metastatic breast cancer

被引：0

作者：

Bo Fu

Ying Zhang

Wei Long

Aifeng Zhang

Yafen Zhang

Yanli An

Fengqin Miao

Fang Nie

Mingli Li

Youji He

Jianqiong Zhang

Gaojun Teng

机构：

[1] Key Laboratory of Developmental Genes and Human Disease,Department of Pathology, Medical School

[2] Ministry of Education; Department of Microbiology and Immunology,undefined

[3] Medical School Southeast University,undefined

[4] Southeast University,undefined

[5] Jiangsu Key Laboratory of Molecular and Functional Imaging,undefined

[6] Department of Radiology,undefined

[7] Zhongda Hospital,undefined

[8] Medical School of Southeast University,undefined

来源：

Biotechnology Letters | 2014年 / 36卷

关键词：

Brain metastatic breast cancer; Cancer cells; Human brain-seeking breast carcinoma cells; Peptide; Phage display; Tumor targeting;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

A novel peptide, BRBP1 (MYPWTEPSYLSN), was identified using an in vitro phage biopanning strategy against human brain-seeking breast carcinoma cells (231-BR cells).The peptide-phage clone, BRBP1-M13 displaying BRBP1 sequence, specifically bound to 231-BR cells and the binding could be competitively abolished by BRBP1. In vivo distribution studies showed that BRBP1-M13 preferentially homed to the 231-BR tumors. Fluorescently-labeled BRBP1, BRBP1-K(5-TAMRA), preferentially bound to 231-BR cells in a dose-dependent and energy-dependent manner and it was efficiently internalized into the cells after 2 h incubation. Near-infrared fluorophores imaging demonstrated the accumulation of Cy5.5-conjugated BRBP1 peptide in the tumors in vivo. Thus, BRBP1 is a promising peptide binding to human brain metastatic breast cancer and it may be applied to targeted delivery of cytotoxic agents to the intended tumor.

引用

页码：2291 / 2301

页数：10

共 122 条

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