FISH Shows That Desulfotomaculum spp. Are the Dominating Sulfate-Reducing Bacteria in a Pristine Aquifer

被引：0
作者：
J. Detmers
H. Strauss
U. Schulte
A. Bergmann
K. Knittel
J. Kuever
机构：
[1] Max-Planck-Institute for Marine Microbiology,Department of Microbiology
[2] Celsiusstrasse 1,Geologisch
[3] D-28359 Bremen,Paläontologisches Institut und Museum
[4] Westfälische Wilhelms-Universität Münster,Institut für Geologie
[5] Corrensstrasse 24,Molecular Ecology
[6] D-48149 Münster,undefined
[7] Ruhr-Universität Bochum,undefined
[8] Universitätsstrasse 150,undefined
[9] D-44801 Bochum,undefined
[10] Rheinisch Westfälisches Institut für Wasser,undefined
[11] Moritzstr. 26,undefined
[12] D-45476,undefined
[13] Mülheim,undefined
[14] Germany,undefined
[15] Max-Planck-Institute for Marine Microbiology,undefined
[16] Celsiusstrasse 1,undefined
[17] D-28359 Bremen,undefined
[18] Department of Microbiology,undefined
[19] Institute for Material Testing,undefined
[20] Foundation Institute for Materials Science,undefined
[21] Paul-Feller-Str. 1,undefined
[22] D-28199 Bremen,undefined
来源：
Microbial Ecology | 2004年 / 47卷
关键词：
Microbial Diversity; Desulfovibrio; Subsurface Environment; Desulfotomaculum; Marine Sand;
D O I：
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中图分类号：
学科分类号：
摘要：
The hydrochemistry and the microbial diversity of a pristine aquifer system near Garzweiler, Germany, were characterized. Hydrogeochemical and isotopic data indicate a recent activity of sulfate-reducing bacteria in the Tertiary marine sands. The community structure in the aquifer was studied by fluorescence in situ hybridization (FISH). Up to 7.3 × 105 cells/mL were detected by DAPI-staining. Bacteria (identified by the probe EUB338) were dominant, representing 51.9% of the total cell number (DAPI). Another 25.7% of total cell were affiliated with the domain Archaea as identified by the probe ARCH915. Within the domain Bacteria, the β-Proteobacteria were most abundant (21.0% of total cell counts). Using genus-specific probes for sulfate-reducing bacteria (SRB), 2.5% of the total cells were identified as members of the genus Desulfotomaculum. This reflects the predominant role these microorganisms have been found to play in sulfate-reducing zones of aquifers at other sites. Previously, all SRB cultured from this site were from the spore-forming genera Desulfotomaculum and Desulfosporosinus.
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页码：236 / 242
页数：6
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