Role of mitochondrial calcium uniporter in regulating mitochondrial fission in the cerebral cortexes of living rats

被引：0
作者：
Nan Liang
Peng Wang
Shilei Wang
Shuhong Li
Yu Li
Jinying Wang
Min Wang
机构：
[1] Affiliated Hospital of Qingdao University Medical College,Department of Anesthesiology
来源：
Journal of Neural Transmission | 2014年 / 121卷
关键词：
Ischemia/reperfusion injury; MCU; Mitochondrial fission; Mitochondrial calcium;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The mitochondrial calcium uniporter (MCU) transports Ca2+ from the cytoplasm to the mitochondrial matrix and thus maintains Ca2+ homeostasis. Previous studies have reported that inhibition of MCU by ruthenium red (RR) protects the brain from ischemia/reperfusion (I/R) injury and that mitochondrial fission plays an important role in I/R injury. However, it is still not known whether MCU affects mitochondrial fission. In the present study, treatment with RR was found to decrease the concentration of free calcium in the mitochondria, calcineurin enzyme activity and dynamin-related protein 1 expression, and treatment with spermine was found to have the opposite effect in organisms subjected to occlusion of the middle cerebral artery lasting 2 h followed by 24 h reperfusion. These results indicate that MCU may be related to mitochondrial fission via modulating mitochondrial Ca2+ uptake and this relationship between MCU and mitochondrial fission may protect the brain from I/R injury.
引用
收藏
页码：593 / 600
页数：7
相关论文
共 95 条
[1]  
Bereiter-Hahn J(1990)Behavior of mitochondria in the living cell Int Rev Cytol 122 1-63
[2]  
Bossy-Wetzel E(2003)Mitochondrial fission in apoptosis, neurodegeneration and aging Curr Opin Cell Biol 15 706-716
[3]  
Barsoum MJ(2008)Dephosphorylation by calcineurin regulates translocation of Drp1 to mitochondria Proc Natl Acad Sci USA 105 15803-15808
[4]  
Godzik A(2010)Inhibition of Drp1-dependent mitochondrial fragmentation and apoptosis by a polypeptide antagonist of calcineurin Cell Death Differ 17 1785-1794
[5]  
Schwarzenbacher R(2007)Regulation of mitochondrial fusion and division Trends Cell Biol 17 563-569
[6]  
Lipton SA(2004)Mitochondrial dynamics in mammals Curr Top Dev Biol 59 119-144
[7]  
Cereghetti GM(2007)Reversible phosphorylation of Drp1 by cyclic AMP-dependent protein kinase and calcineurin regulates mitochondrial fission and cell death EMBO Rep 8 939-944
[8]  
Stangherlin A(2004)Cell death: critical control points Cell 116 205-219
[9]  
de Brito OM(2006)(De)constructing mitochondria: what for? Physiology (Bethesda) 21 233-241
[10]  
Cereghetti GM(1996)Apoptosis in ischemic and reperfused rat myocardium Circ Res 79 949-956
12345678910