High-quality draft genome sequence and description of Haemophilus massiliensis sp nov.

被引:33
|
作者
Lo, Cheikh Ibrahima [1 ,2 ]
Sankar, Senthil Alias [1 ]
Fall, Becaye [3 ]
Sambe-Ba, Bissoume [3 ]
Diawara, Silman [3 ]
Gueye, Mamadou Wague [3 ]
Mediannikov, Oleg [1 ,2 ]
Blanc-Tailleur, Caroline [1 ]
Wade, Boubacar [3 ]
Raoult, Didier [1 ,2 ,4 ]
Fournier, Pierre-Edouard [1 ]
Fenollar, Florence [1 ,2 ]
机构
[1] Aix Marseille Univ, URMITE, Fac Med, UM63,CNRS 7278,IRD 198,Inserm U1095, Marseille, France
[2] Campus Int UCAD IRD, Dakar, Senegal
[3] Hop Principal, Dakar, Senegal
[4] King Abdulaziz Univ, King Fahd Med Res Ctr, Special Infect Agents Unit, Jeddah 21413, Saudi Arabia
来源
关键词
Haemophilus massiliensis; Genome; Taxono-genomics; Culturomics; DNA-DNA HYBRIDIZATION; BACTERIA; IDENTIFICATION; COMMITTEE; ALIGNMENT; PROPOSAL;
D O I
10.1186/s40793-016-0150-1
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Strain FF7(T) was isolated from the peritoneal fluid of a 44-year-old woman who suffered from pelvic peritonitis. This strain exhibited a 16S rRNA sequence similarity of 94.8 % 16S rRNA sequence identity with Haemophilus parasuis, the phylogenetically closest species with a name with standing in nomenclature and a poor MALDI-TOF MS score (1.32 to 1.56) that does not allow any reliable identification. Using a polyphasic study made of phenotypic and genomic analyses, strain FF7(T) was a Gram-negative, facultatively anaerobic rod and member of the family Pasteurellaceae. It exhibited a genome of 2,442,548 bp long genome (one chromosome but no plasmid) contains 2,319 protein-coding and 67 RNA genes, including 6 rRNA operons. On the basis of these data, we propose the creation of Haemophilus massiliensis sp. nov. with strain FF7(T) (= CSUR P859 = DSM 28247) as the type strain.
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页数:9
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