Anti-diabetic Effect of Boerhavia diffusa L. Root Extract via Free Radical Scavenging and Antioxidant Mechanism

被引：11

作者：

Alam P. ^{[1
]}

Shahzad N. ^{[2
]}

Gupta A.K. ^{[3
]}

Mahfoz A.M. ^{[2
]}

Bamagous G.A. ^{[2
]}

Al-Ghamdi S.S. ^{[2
]}

Siddiqui N.A. ^{[1
]}

机构：

[1] Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh

[2] Department of Pharmacology and Toxicology, Faculty of Medicine, Umm Al-Qura University, Makkah

[3] Department of Pharmacognosy and Phytochemistry, SBS College of Pharmacy, Chuslewal Morh Patti, Punjab

来源：

Toxicology and Environmental Health Sciences | 2018年 / 10卷 / 3期

关键词：

Antioxidants; Boerhavia diffusa; Diabetes mellitus; Free radicals; Nitric oxide; SGOT;

D O I：

10.1007/s13530-018-0367-z

中图分类号：

学科分类号：

摘要：

Objective: The present study was proposed to assess the in vitro free radical-scavenging activity of B. diffusa methanolic extract (BDME) and its modulatory effect against streptozotocin (STZ) induced diabetes in male Wistar rats. Methods: Experimental diabetes was induced in Wistar albino rats by administering single dose of STZ 40 mg/kg. One week later rats with blood glucose level >200 mg/dL were segregated as diabetes in three groups each containing 6 rats in number. Results: Total phenolic content in B. diffusa methanol extract (BDME) was found to be 87 mg of gallic acid equivalents/g extract and total flavonoid content found to be 54.1 mg of quercetin equivalents/g extract. Its extract also exhibited DPPH (IC50, 163.1±6.7 μg/mL), nitric oxide (295 μg/mL) and H2O2 (159±5.25 μg/mL) radical scavenging activity. Pre-treatment with BDME (100 and 200 mg/kg b.w.) in streptozotocin-induced diabetic rats resulted in significant improvement in blood glucose, blood plasma enzymes SGOT, SGPT and ALP, weight loss, total protein, serum insulin and liver glycogen levels. Furthermore, it restores the activity of antioxidant enzymes viz. SOD, CAT and GPx. Conclusion: Thus, the result suggests that BDME employed significant anti-diabetic effect in Wistar rats which is associated with its free radical scavenging and antioxidant activity. © 2018, Korean Society of Environmental Risk Assessment and Health Science and Springer Nature B.V.

引用

页码：220 / 227

页数：7

共 50 条

[1]

Umar A., Ahmed Q.U., Muhammad B.Y., Dogarai B.B., Soad S.Z., Anti-hyperglycemic activity of the leaves of Tetracera scandens Linn. Merr. (Dille-niaceae) in alloxan induced diabetic rats, J. Ethnopharmacol, 1, pp. 140-145, (2010)

[2]

Wild S.G., Roglic A., Green R., Sicree R., King H., Global prevalence of diabetes: estimated for the year 2000 and projection for 2030, Diabetes Care, 5, pp. 1047-1053, (2004)

[3]

Dewanjee S., Das A.K., Sahu R., Gangopadhyay M., Antidiabetic activity of Diospyros peregrine fruit: effect on hyperglycemia, hyperlipidemia and augmented oxidative stress in experimental type 2 diabetes, Food Chem. Toxicol, 47, pp. 2679-2685, (2009)

[4]

Ivorra M.D., Paya M., Villar A., A review of natural products and plants as potent antidiabetic drugs, J. Ethnopharmacol, 27, pp. 243-276, (1989)

[5]

Rendel A.B., The Classification of Flowering Plants. Dicotyledons, (1925)

[6]

Mitra R., Gupta R.C., Punarnava -an Ayurvedic drug of repute. Applied Botany Abstracts, (1997)

[7]

Olukoya D.K., Tdika N., Odugbemi T., Antibacterial activity of some medicinal plants from Nigeria, J. Ethnopharmacol, 39, pp. 69-72, (1993)

[8]

Adesina S.K., Anticonvulsant properties of the root bark of Boerhavia diffusa, J. Crude Drug Res, 17, pp. 84-86, (1979)

[9]

Bhalla T.N., Gupta M.B., Sheth P.K., Bhargava K.P., Anti-inflammatory activity of Boerhaavia diffusa, Indian. J. Physiol. Pharmacol, 12, (1968)

[10]

Vijayalakshmi K., Misra S.D., Prasad S.K., Nematicidal properties of some indigenous plant materials against second stage juveniles of Meloidogyne incognita (Kofoid and White) Chitwood, Indian J. Entomol, 41, pp. 326-331, (1979)

← 1 2 3 4 5 →