Human adipose tissue derived mesenchymal stem cells are resistant to several chemotherapeutic agents

被引：0
作者：
Wei Liang
Hailong Xia
Jing Li
Robert Chunhua Zhao
机构：
[1] The First Affiliated Hospital of Anhui Medical University,Department of Oncology, Center for Cell Therapy and Tissue Engineering
[2] Chinese Academy of Medical Science and Peking Union Medical College,Institute of Basic Medical Science and School of Basic Medicine, Center of Excellence in Tissue Engineering
来源：
Cytotechnology | 2011年 / 63卷
关键词：
Adipose tissue; MSCs; Chemoresistance; Cisplatin; Vincristine; Camptothecin;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Human adipose derived mesenchymal stem cells (ADMSCs) are multipotential stem cells, originated from the vascular stromal compartment of fat tissues which can be used as an alternative cell source for many different cell therapies. However, their response to chemotherapeutic agants remains unknown. Here we assessed the acute direct effects of individual chemotherapeutic drug on ADMSCs. Using an in vitro culture system, the response of ADMSCs to the three chemotherapeutic agents cisplatin, comptothecin and vincristine was determined in comparison with that of testicular germ cell tumour (TGCT) cell line. The recovery of cell numbers following exposure to chemotherapeutic agents were also evaluated. Our results showed that human ADMSCs were resistant to chemo-therapeutic agents which are commonly used in clinic, the full recovery was seen respectively in ADMSCs after the drug treatment. Moreover, ADMSCs maintained their stem cell characteristics in vitro after the exposure to all chemotherapeutic agents.
引用
收藏
页码：523 / 530
页数：7
相关论文
共 171 条
[11]  
Meng Y(1999)Marrow stromal cells migrate throughout forebrain and cerebellum, and they differentiate into astrocytes after injection into neonatal mouse brains Proc Natl Acad Sci USA 96 10711-10716
[12]  
Han Q(2004)Differential damage and recovery of human mesenchymal stem cells after exposure to chemotherapeutic agents Br J Haematol 127 326-334
[13]  
Zhao RC(2000)Human mesenchymal stem cells engraft and demonstrate site-specific differentiation after in utero transplantation in sheep Nat Med 6 1282-1286
[14]  
Chen MF(2006)Presence of mesenchymal stem cells in human bone marrow after exposure to chemotherapy: evidence of resistance to apoptosis induction Stem Cells (Dayton, Ohio) 24 2753-2765
[15]  
Lin CT(2003)In vivo and in vitro differentiation of myocytes from human bone marrow-derived multipotent progenitor cells Exp Hematol 31 1323-1330
[16]  
Chen WC(2000)Clonal mesenchymal progenitors from human bone marrow differentiate in vitro according to a hierarchical model J Cell Sci 113 1161-1166
[17]  
Yang CT(2003)Mesenchymal stem cell injection induces cardiac nerve sprouting and increased tenascin expression in a Swine model of myocardial infarction J Cardiovasc Electrophysiol 14 841-848
[18]  
Chen CC(2004)Susceptibility of human fetal mesenchymal stem cells to Kaposi sarcoma-associated herpesvirus Blood 104 2736-2738
[19]  
Liao SK(1998)Marrow stromal cells as a source of progenitor cells for nonhematopoietic tissues in transgenic mice with a phenotype of osteogenesis imperfecta Proc Natl Acad Sci USA 95 1142-1147
[20]  
Liu JM(1999)Multilineage potential of adult human mesenchymal stem cells Science 284 143-147
12345678910