Genome-wide analysis of the bHLH gene family in Chinese jujube (Ziziphus jujuba Mill.) and wild jujube

被引：13

作者：

Li H. ^{[1
,2
]}

Gao W. ^{[1
,2
]}

Xue C. ^{[1
,2
]}

Zhang Y. ^{[1
,2
]}

Liu Z. ^{[3
]}

Zhang Y. ^{[1
,2
]}

Meng X. ^{[3
]}

Liu M. ^{[3
]}

Zhao J. ^{[1
,2
]}

机构：

[1] College of Life Science, Hebei Agricultural University, Baoding

[2] Hebei Key Laboratory of Plant Physiology and Molecular Pathology, Hebei Agricultural University, Baoding

[3] Research Center of Chinese Jujube, Hebei Agricultural University, Baoding

[4] College of Forestry, Hebei Agricultural University, Baoding

来源：

BMC Genomics | / 20卷 / 1期

基金：

中国国家自然科学基金;

关键词：

Chinese jujube; Flower and fruit development; Phytoplasma; Protein-protein interaction; Tissue-specific expression; ZjbHLHs;

D O I：

10.1186/s12864-019-5936-2

中图分类号：

学科分类号：

摘要：

Background: The bHLH (basic helix-loop-helix) transcription factor is one of the largest families of transcription factors in plants, containing a large number of members with diverse functions. Chinese jujube (Ziziphus jujuba Mill.) is the species with the highest economic value in the family Rhamnaceae. However, the characteristics of the bHLH family in the jujube genome are still unclear. Hence, ZjbHLHs were first searched at a genome-wide level, their expression levels under various conditions were investigated systematically, and their protein-protein interaction networks were predicted. Results: We identified 92 ZjbHLHs in the jujube genome, and these genes were classified into 16 classes according to bHLH domains. Ten ZjbHLHs with atypical bHLH domains were found. Seventy ZjbHLHs were mapped to but not evenly distributed on 12 pseudo- chromosomes. The domain sequences among ZjbHLHs were highly conserved, and their conserved residues were also identified. The tissue-specific expression of 37 ZjbHLH genes in jujube and wild jujube showed diverse patterns, revealing that these genes likely perform multiple functions. Many ZjbHLH genes were screened and found to be involved in flower and fruit development, especially in earlier developmental stages. A few genes responsive to phytoplasma invasion were also verified. Based on protein-protein interaction prediction and homology comparison, protein-protein interaction networks composed of 92 ZjbHLHs were also established. Conclusions: This study provides a comprehensive bioinformatics analysis of 92 identified ZjbHLH genes. We explored their expression patterns in various tissues, the flowering process, and fruit ripening and under phytoplasma stress. The protein-protein interaction networks of ZjbHLHs provide valuable clues toward further studies of their biological functions. © 2019 The Author(s).

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