Effect of culture conditions on growth and production of docosahexaenoic acid (DHA) usingThraustochytrium aureum ATCC 34304

被引：27
作者：
Byung-Ki Hur
Dae-Won Cho
Ho-Jung Kim
Chun-Ik Park
Hyung-Joon Suh
机构：
[1] Inha University,Department of Biotechnology and Bioengineering
[2] Inha University,Department of Environmental Engineering
来源：
Biotechnology and Bioprocess Engineering | 2002年 / 7卷 / 1期
关键词：
DHA production; environmental factors; glucose concentration effect;
D O I：
10.1007/BF02935873
中图分类号：
学科分类号：
摘要：
Environmental and medium factors were investigated as basic data for optimizing DHA production when usingThraustochytrium aureum. To study the effect of environmental conditions, the rotation speed and culture temperature were, changed. Plus the trend of the growth characteristics, lipid content in the biomass, and DHA content in lipids were evaluated according to various initial glucose concentrations. The biomass, lipid, and DHA analyses showed that the physiological characteristics ofT. aureum were closely related with the environmental and medium conditions, as in the case of other marine microorganisms. For example, a low rotation speed of 50 rpm lowered the cell growth rate as well as the DHA content in the lipids. A low temperature had a negative effect on the cell growth, yet a positive effect on the lipid content in the biomass. Different initial glucose concentrations had no effect on the lipid content in the biomass or DHA content in the lipids, yet did affect the cell growth. Accordingly, these results show that environmental and medium factors must be synthetically considered in order to optimize DHA production when usingT. aureum.
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页码：10 / 15
页数：5
相关论文
共 64 条
[1]  
Singh A.(1996)Production of high yields of docosahexaenoic acid by J. Ind. Microbiol. 12 370-373
[2]  
Ward O. P.(1995) ATCC 28210 INFORM 6 683-688
[3]  
Ward O. P.(1986)Microbial production of long-chain PUFAs Prog. Lipid Res. 25 595-606
[4]  
Bazan N. G.(1990)Metabolism of arachidonic and docosahexaenoic acid in the retina Pediar. Res. 28 485-492
[5]  
Reddy T. S.(1990)Effect of dietary omega-3 fatty acids on retinal function of very-low-brith-weight neonates J. Lipid Res. 31 237-247
[6]  
Bazan H. E. P.(1991)Dietary effects on brain fatty acid composition, the reversibility of n-3 fatty acid deficiency and turnover of doecosahexaenoic acid in brain, erythrocyte and plasma of rhesus monkey JAOCS 68 509-514
[7]  
Brikle D. L.(1975)Optimization of production of docosahexaenoic acid (DHA) by Lipids 10 395-403
[8]  
Uauy R. D.(1966) ATCC 34304 Biochem. Biophys. Acta 116 577-579
[9]  
Brich D. G.(1977)Identification of 3, 6, 9, 12, 15-octadecapentaenoic acid in laboratory-cultured photosynthetic dinoflagellates Lipids 12 989-993
[10]  
Brich E. Z.(1967)Fatty acids of the ‘red tide’ organism Can. J. Microbiol. 13 755-760
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