Effects of reduced shear stress on inflammatory reactions in vitro. Effects of pathological flow conditions on leukocyte-endothelial interactions and monocyte tissue factor expression in human cell cultures

被引：2
作者：
Nohé B. [1 ,4 ]
Johannes T. [1 ,2 ]
Schmidt V. [3 ]
Schroeder T.H. [1 ]
Kiefer R.T. [1 ]
Unertl K. [1 ]
Dieterich H.J. [1 ]
机构：
[1] Abteilung für Anaesthesiologie und Intensivmedizin, Universitätsklinikum Tübingen
[2] Department of Physiology, Academic Medical Center, University of Amsterdam
[3] II. Chirurgische Klinik, Klinikum Augsburg
[4] Abteilung für Anaesthesiologie und Intensivmedizin, Universitätsklinikum, 72076 Tübingen
来源：
Der Anaesthesist | 2005年 / 54卷 / 8期
关键词：
Endothelium; Inflammation; Leukocytes; Shear stress; Tissue factor;
D O I：
10.1007/s00101-005-0852-z
中图分类号：
学科分类号：
摘要：
Background. During malperfusion and inflammation leukocyte adhesion is common. The purpose of this study was to examine the effects of reduced shear stress on leukocyte-endothelial interactions and subsequent inflammatory reactions such as up-regulation of tissue factor. Methods. Isolated neutrophils and monocytes were co-incubated with human umbilical venous endothelium at 0-3 dynes/cm 2 in a flow chamber. Adhesion and tissue factor expression on adherent leukocytes were examined at various flow conditions. Results. At 2-3 dynes/cm 2 adhesion occurred only on TNFα-activated endothelium. Below 1 dyne/cm 2 similarly increased adhesion was also observed on non-activated endothelium. As was observed for leukocyte adhesion, these shear stress-dependent cell interactions also resulted in an up-regulation of tissue factor on adherent monocytes from non-activated co-cultures. Conclusion. Apart from additional activators of inflammation, reduced shear forces may directly contribute to inflammation. © Springer Medizin Verlag 2005.
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页码：773 / 780
页数：7
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