Expression of the Commelina yellow mottle virus promoter in transgenic oat

被引：0

作者：

K. A. Torbert

M. Gopalraj

S. L. Medberry

N. E. Olszewski

D. A. Somers

机构：

[1] Department of Agronomy and Plant Genetics,

[2] 411 Borlaug Hall 1991 Upper Buford Circle,undefined

[3] University of Minnesota,undefined

[4] St. Paul,undefined

[5] MN55108,undefined

[6] USA,undefined

[7] Department of Plant Biology,undefined

[8] Plant Molecular Genetics Institute,undefined

[9] University of Minnesota,undefined

[10] St. Paul,undefined

[11] MN 55108,undefined

[12] USA,undefined

来源：

Plant Cell Reports | 1998年 / 17卷

关键词：

Key words Transgenic; Promoter; Badnavirus; Oat; Avena;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

The Commelina yellow mottle virus (CoYMV) infects the monocot weed Commelina diffusa. The objective of this study was to investigate the transgene expression conferred by the CoYMV promoter in a monocot species. Friable, embryogenic oat (Avena sativa L.) tissue cultures were stably transformed with the CoYMV promoter fused to the coding region of E. coli β-glucuronidase (uidA, GUS). Developmental and tissue-specific expression of the CoYMV-GUS construct was investigated in regenerated plants and their progeny. Histochemical GUS staining was primarily localized in the vascular tissues of shoots, leaves, floral bracts and in roots. While ovaries stained intensely, no staining was detected in anthers or the endosperm in mature seed. The scutellum of mature and germinating seed exhibited GUS activity.

引用

页码：284 / 287

页数：3