Quality of tumor lysates used for pulsing dendritic cells is influenced by the method used to harvest adherent tumor cells

被引：5

作者：

Herzog G.I. ^{[1
]}

Solgi G. ^{[3
]}

Wiegmann D.S. ^{[1
]}

Nienhaus C. ^{[1
]}

Schrezenmeier H. ^{[1
,2
]}

Yildiz T. ^{[1
]}

Lotfi R. ^{[1
,2
]}

机构：

[1] Institute of Transfusion Medicine, University of Ulm

[2] Institute of Clinical Transfusion Medicine and Immunogenetics Ulm, German Red Cross Blood Services Baden-Württemberg-Hessen, 89081 Ulm

[3] Immunology Department, School of Medicine, Ahvaz Jundishapur University of Medical Sciences (AJUMS)

来源：

BMC Research Notes | / 4卷 / 1期

关键词：

Trypsin; Tumor Cell Lysate; Standard Cell Culture; Maturation Marker; Standard Cell Culture Condition;

D O I：

10.1186/1756-0500-4-153

中图分类号：

学科分类号：

摘要：

Background: Lysates from tumor cells are reported to induce maturation of dendritic cells (DCs) and are used in clinical settings for DC-based vaccination against solid tumors. Nevertheless, the maturation inducing effect of tumor lysates on DCs is discussed controversially and the efficacy of tumor vaccines varies significantly. Findings. Using three individual adherent colorectal tumor cell lines we also faced the difficulty to obtain consistent results regarding maturation inducing effect of tumor lysates on DCs. Therefore, we compared different methods to prepare tumor cell lysate and could demonstrate that trypsinizing as a method to harvest adherent tumor cells has a significant negative impact on biologic activity of tumor lysates. Specifically, we assessed induction of maturation markers CD40, CD80, and CD86 on DCs which were treated with differently prepared lysates. Conclusions: Trypsinizing is a very common way of harvesting adherent cells from culture flasks. Our results shall call investigators' attention to the enzymatic activity of trypsin degrading some possibly important proteins on the surface of cultured cells. Specifically for DC-based vaccination against tumor antigens investigators should avoid trypsin. © 2011 Lotfi et al; licensee BioMed Central Ltd.

引用

共 6 条

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[4] Toh H.C., Wang W.W., Chia W.K., Kvistborg P., Sun L., Teo K., Et al., Clinical Benefit of Allogeneic Melanoma Cell Lysate-Pulsed Autologous Dendritic Cell Vaccine in MAGE-Positive Colorectal Cancer Patients, Clin Cancer Res, 15, 24, pp. 7726-7736, (2009)
[5] Segura-Egea J.J., Jimenez-Rubio A., Rios-Santos J.V., Velasco-Ortega E., Calvo-Gutierrez J.R., In vitro inhibitory effect of EGTA on macrophage adhesion: Endodontic implications, Journal of Endodontics, 29, 3, pp. 211-213, (2003)
[6] Lotfi R., Lotze M.T., Eosinophils induce DC maturation, regulating immunity, Journal of Leukocyte Biology, 83, 3, pp. 456-460, (2008)

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